EC Number   |
|---|
   2.7.11.8 | HA-tagged FAST K overexpression in HeLa cells enhances exon 6 inclusion of Fas reporters transfected in the cells, effects of FAST K are mediated by changes in the function of TIA-1/TIAR, i.e. T-cell intracellular antigen 1, and TIA-related proteins, the effects of FAST K overexpression are largely suppressed by depletion of TIA-1 and TIAR and are significantly compromised by mutation of a TIA-1/TIAR-responsive enhancer present downstream of exon 6 5' splice site, expression of GST-tagged TIA in HeLa cells |
| 2.7.11.8 | kinase domain of FASTK353-444 is cloned in pET28a, and the recombinant FASTK is expressed in Escherichia coli BL21 (DE3) cells |
| 2.7.11.8 | overexpression of HA-tagged enzyme in HeLa cells and in COS-7 cells, expression of GFP-tagged enzyme in COS-7 cells |
| 2.7.11.8 | overexpression of HA-tagged enzyme in HeLa cells and in COS-7 cells, recombinant FAST promotes the expression of co-transfected reporter proteins, e.g. beta-galactosidase, via its TIA-1 binding domain, and increases the expression of endogenous cIAP-1 and XIAP, but not GAPDH, in HeLa cells |
