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EC Number Cloned (Commentary)
Show all pathways known for 2.7.1.178Display the reaction diagram Show all sequences 2.7.1.178expression in Escherichia coli
Show all pathways known for 2.7.1.178Display the reaction diagram Show all sequences 2.7.1.178produced as inclusion bodies in Escherichia coli when polyhistidine is used as a fusion tag. To reduce inclusion body formation in Escherichia coli, the enzyme is fused with three partners, thioredoxin, glutathione-S-transferase, and N-utilization substance A. With the use of fusion-partners, the solubility of the archaeal protein is remarkably enhanced, and the soluble fraction of the recombinant protein is increased in this order: thioredoxin > glutathione-S-transferase > N-utilization substance A. In the case of recombinant enzyme, the enzyme activity of the Trx-fused protein is 200-fold higher than that of the polyhistidine-fusion protein
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