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IUBMB Comments The enzyme from the archaeon Sulfolobus solfataricus is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It phosphorylates 2-dehydro-3-deoxy-D -gluconate and 2-dehydro-3-deoxy-D -galactonate with similar catalytic efficiency. cf . EC 2.7.1.45 , 2-dehydro-3-deoxygluconokinase and EC 2.7.1.58 , 2-dehydro-3-deoxygalactonokinase.
The expected taxonomic range for this enzyme is: Archaea, Bacteria
Synonyms 2-keto-3-deoxy-D-gluconate kinase, 2-keto-3-deoxygalactonate kinase, 2-keto-3-deoxygluconate kinase, 2-keto-3-deoxyglucononate kinase, HVO_0549, HVO_A0328, KDG kinase, KDGK, KDGK-1, KDGK-2, more
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2-keto-3-deoxy-D-gluconate kinase
2-keto-3-deoxygalactonate kinase
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2-keto-3-deoxygluconate kinase
2-keto-3-deoxyglucononate kinase
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2-keto-3-deoxy-D-gluconate kinase
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2-keto-3-deoxy-D-gluconate kinase
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2-keto-3-deoxygluconate kinase
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2-keto-3-deoxygluconate kinase
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2-keto-3-deoxygluconate kinase
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KDG kinase
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KDGK
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SSO3195
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ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
(2)
ATP + 2-dehydro-3-deoxy-D-galactonate = ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
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ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
(1)
ATP + 2-dehydro-3-deoxy-D-gluconate = ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
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MetaCyc
3,6-anhydro-alpha-L-galactopyranose degradation, 4-deoxy-L-threo-hex-4-enopyranuronate degradation, alginate degradation, D-fructuronate degradation, D-galactonate degradation, D-galacturonate degradation I, Entner-Doudoroff pathway III (semi-phosphorylative), L-glucose degradation
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ATP:2-dehydro-3-deoxy-D-gluconate/2-dehydro-3-deoxy-D-galactonate 6-phosphotransferase
The enzyme from the archaeon Sulfolobus solfataricus is involved in glucose and galactose catabolism via the branched variant of the Entner-Doudoroff pathway. It phosphorylates 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate with similar catalytic efficiency. cf. EC 2.7.1.45, 2-dehydro-3-deoxygluconokinase and EC 2.7.1.58, 2-dehydro-3-deoxygalactonokinase.
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ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
Substrates: - Products: -
?
ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
additional information
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ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
Substrates: 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
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Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: 2-dehydro-3-deoxy-D-gluconate and 2-dehydro-3-deoxy-D-galactonate are phosphorylated with similar catalytic efficiency Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway Products: -
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additional information
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Substrates: no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate Products: -
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additional information
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Substrates: no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate Products: -
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ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-6-phospho-D-galactonate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-galactonate
ADP + 2-dehydro-3-deoxy-D-galactonate 6-phosphate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
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Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 2-dehydro-3-deoxy-6-phospho-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: - Products: -
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ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: a key enzyme in the semiphosphorylative Entner-Doudoroff pathway in archaea Products: -
?
ATP + 2-dehydro-3-deoxy-D-gluconate
ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate
Substrates: the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway Products: -
?
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8.1
2-dehydro-3-deoxy-D-galactonate
pH 7.5, 60°C
0.14 - 3.6
2-dehydro-3-deoxy-D-gluconate
0.14
2-dehydro-3-deoxy-D-gluconate
pH 7.2, 60°C
3.6
2-dehydro-3-deoxy-D-gluconate
pH 7.5, 60°C
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5.4
2-dehydro-3-deoxy-D-galactonate
pH 7.5, 60°C
5 - 60.8
2-dehydro-3-deoxy-D-gluconate
5
2-dehydro-3-deoxy-D-gluconate
pH 7.5, 60°C
60.8
2-dehydro-3-deoxy-D-gluconate
pH 7.2, 60°C
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0.7
2-dehydro-3-deoxy-D-galactonate
pH 7.5, 60°C
1.4 - 434
2-dehydro-3-deoxy-D-gluconate
1.4
2-dehydro-3-deoxy-D-gluconate
pH 7.5, 60°C
434
2-dehydro-3-deoxy-D-gluconate
pH 7.2, 60°C
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6.1
calculated from sequence
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brenda
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UniProt
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SwissProt
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brenda
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SwissProt
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SwissProt
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SwissProt
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Highest Expressing Human Cell Lines
Filter by:
Cell Line Links
Gene Links
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metabolism
KDGK-1 represents the functional constitutively expressed 2-keto-3-deoxygluconate kinase in glucose degradation, whereas KDGK-2 is an inducible 2-keto-3-deoxygalactonate kinase likely involved in D-galactose catabolism
malfunction
a 2-keto-3-deoxygluconate kinase deletion strain reveals no difference in glycolytic growth compared with the reference strain
malfunction
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a 2-keto-3-deoxygluconate kinase deletion strain reveals no difference in glycolytic growth compared with the reference strain
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KDGK_SACS2
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
313
0
34875
Swiss-Prot
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KDGK1_HALVD
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
320
0
34083
Swiss-Prot
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A0A1W6VRI4_GEOTD
316
0
34595
TrEMBL
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A0A871BC54_HALGI
318
0
33417
TrEMBL
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A0A0C7NJK1_DEFTU
316
0
34799
TrEMBL
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A0A5B9DEQ6_9ARCH
345
0
38489
TrEMBL
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A0A650CJU4_SULOH
311
0
34529
TrEMBL
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KDGK2_HALVD
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2)
318
0
33998
Swiss-Prot
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34874
2 * 34874, calculated from sequence
34875
x * 34875, calculated from sequence
37513
x * 37513, mass spectrometric analysis
43000
2 * 43000, SDS-PAGE
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?
x * 34875, calculated from sequence
?
x * 37513, mass spectrometric analysis
homodimer
2 * 43000, SDS-PAGE
homodimer
2 * 34874, calculated from sequence
homodimer
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2 * 43000, SDS-PAGE
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homodimer
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2 * 34874, calculated from sequence
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sitting drop vapour diffusion method, crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apoenzyme is solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-dehydro-3-deoxygluconate and an ATP analogue is resolved at 2.1 A. The complex suggests that the structural basis for the enzymes ability to phosphorylate 2-oxo-3-deoxygluconate and 2-dehydro-3-deoxygalactonate is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases
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HiPrep column chromatography and Sephacryl S-200 gel filtration
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expression in Escherichia coli
produced as inclusion bodies in Escherichia coli when polyhistidine is used as a fusion tag. To reduce inclusion body formation in Escherichia coli, the enzyme is fused with three partners, thioredoxin, glutathione-S-transferase, and N-utilization substance A. With the use of fusion-partners, the solubility of the archaeal protein is remarkably enhanced, and the soluble fraction of the recombinant protein is increased in this order: thioredoxin > glutathione-S-transferase > N-utilization substance A. In the case of recombinant enzyme, the enzyme activity of the Trx-fused protein is 200-fold higher than that of the polyhistidine-fusion protein
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Kim, S.; Lee, S.B.
Characterization of Sulfolobus solfataricus 2-keto-3-deoxy-D-gluconate kinase in the modified Entner-Doudoroff pathway
Biosci. Biotechnol. Biochem.
70
1308-1316
2006
Saccharolobus solfataricus (Q97U29), Saccharolobus solfataricus P2 (Q97U29)
brenda
Kim, S.; Lee, S.B.
Soluble expression of archaeal proteins in Escherichia coli by using fusion-partners
Protein Expr. Purif.
62
116-119
2008
Saccharolobus solfataricus (Q97U29)
brenda
Potter, J.A.; Kerou, M.; Lamble, H.J.; Bull, S.D.; Hough, D.W.; Danson, M.J.; Taylor, G.L.
The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase
Acta Crystallogr. Sect. D
64
1283-1287
2008
Saccharolobus solfataricus (Q97U29)
brenda
Lamble, H.J.; Theodossis, A.; Milburn, C.C.; Taylor, G.L.;, Bull, S.D.; Hough, D.W.; Danson, M.J.
Promiscuity in the part-phosphorylative Entner-Doudoroff pathway of the archaeon Sulfolobus solfataricus
FEBS Lett.
579
6865-6869
2005
Saccharolobus solfataricus (Q97U29)
brenda
Kouril, T.; Wieloch, P.; Reimann, J.; Wagner, M.; Zaparty, M.; Albers, S.V.; Schomburg, D.; Ruoff, P.; Siebers, B.
Unraveling the function of the two Entner-Doudoroff branches in the thermoacidophilic crenarchaeon Sulfolobus solfataricus P2
FEBS J.
280
1126-1138
2013
Saccharolobus solfataricus, Saccharolobus solfataricus (Q97U29), Saccharolobus solfataricus P2, Saccharolobus solfataricus P2 (Q97U29)
brenda
Ji, S.; Wang, B.; Lu, M.; Li, F.
Defluviitalea phaphyphila sp. nov., a novel thermophilic bacterium that degrades brown algae
Appl. Environ. Microbiol.
82
868-877
2015
Defluviitalea phaphyphila
brenda
Pickl, A.; Johnsen, U.; Archer, R.; Schnheit, P.
Identification and characterization of 2-keto-3-deoxygluconate kinase and 2-keto-3-deoxygalactonate kinase in the haloarchaeon Haloferax volcanii
FEMS Microbiol. Lett.
361
76-83
2014
Haloferax volcanii (D4GR05), Haloferax volcanii (D4GSE6)
brenda
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