EC Number   |
|---|
    2.5.1.47 | - |
| 2.5.1.47 | construction of transgenic Nicotiana tabacum carrying either spinach cytosolic cDNA, designated 3F plants, or chimeric CSAse A cDNA fused with the sequence for chloroplast-targeting transit peptide of pea Rubisco small subunit, designated 4F plants. Generation of F1 transgenic tobacco, highly tolerant to sulfur-containing pollutants, in which Csase activities are enhanced both in cytosol and in the chloroplasts by crossing 3F plants with 4F plants |
| 2.5.1.47 | cotyledon segments of seedlings of Ipomoea aquatica are transformed with Arabidopsis serine acetyltransferase gene and Oryza sativa cysteine synthase gene under the control of the cauliflower mosaic virus 35S promoter. Strengthening of serine acetyltransferase and cysteine synthase results in increase not only in sulfate uptake, but also in total biomass |
| 2.5.1.47 | DNA and amino acid sequence determination and analysis, sequence comparisons |
| 2.5.1.47 | expressed as a GST-fusion protein |
| 2.5.1.47 | expressed in Escherichia coli |
| 2.5.1.47 | expressed in Escherichia coli as a His-tagged fusion protein |
| 2.5.1.47 | expressed in Escherichia coli as GST-fusion proteins |
| 2.5.1.47 | expressed in Escherichia coli BL21(DE3) cells |
| 2.5.1.47 | expressed in Escherichia coli NM522 with plasmid pRSM40 |
