EC Number |
---|
2.4.3.2 | - |
2.4.3.2 | a soluble form of hST3Gal II is expressed in COS-7 cells |
2.4.3.2 | expressed in 293FT cells |
2.4.3.2 | expressed in CHO cells and and HEK-293T cells |
2.4.3.2 | expressed in COS-7 cells |
2.4.3.2 | expressed in COS-7 cells as a recombinant enzyme fused with protein A |
2.4.3.2 | expressed in HEK-293 cells |
2.4.3.2 | expression in CHO-K1 cells of truncated protein-A-His-tagged enzyme |
2.4.3.2 | expression in COS cells |
2.4.3.2 | expression in COS-7 cell line of various constructs deleted in the N-terminal portion of the protein sequence. The souble forms of the protein consisting of amino acids 26-340, hST3-DELTA25, and amino acids 57-340, hST3-DELTA56, are efficiently secreted and active. Further deletion of the N-terminal region gives also rise to various polypeptides that are not active within the transfected cells and not secreted in the culture medium |