EC Number   |
|---|
    2.4.1.83 | - |
| 2.4.1.83 | capillary endothelial cell clone overexpressing the gene encoding DPMS |
| 2.4.1.83 | cDNA fragments containing the start and stop codons amplified and subcloned into the EcoRV site of the pBluescript II SK(-) cloning vector |
| 2.4.1.83 | DPM1 cDNA from wild-type and Lec15.1 cells is identical |
| 2.4.1.83 | DPMS (KB-3) gene cloned into the pcDNA3.1/His A vector at the KpnI and XhoI site and transformed into DH5alpha cells. Cloned into Xho I and BamH I sites of pEGFP-N1 to obtain pEGFP-N1-DPMS overexpression plasmid. Transfection of capillary endothelial cells with pEGFP-N1-DPMS overexpression plasmid and pEGFP-N1 vector |
| 2.4.1.83 | expressed in Escherichia coli |
| 2.4.1.83 | expressed in Trichoderma atroviride strain P1 |
| 2.4.1.83 | full-length DPMS expressed in Escherichia coli |
| 2.4.1.83 | full-length protein is expressed in Escherichia coli and the N-terminal domain is expressed in Saccharomyces cerevisiae |
| 2.4.1.83 | functionally active mutant enzyme S141A is expressed in Escherichia coli |
