EC Number   |
|---|
   1.14.20.4 | - |
| 1.14.20.4 | a full-length, 1427-bp long cDNA named RnANS1 (Ribes nigrum anthocyanidin synthase 1), which is homologous to the anthocyanidin synthase gene, is cloned from blackcurrant using a homologous cloning strategy. RnANS1 is highly homologous to other plant ANS genes at both the nucleotide and amino acid sequence levels. The deduced protein contains domains conserved in the 2-oxoglutarate and Fe(II)-dependent oxygenase, and is phylogenetically closely related to Paeonia suffruticosa and Paeonia lactiflora |
| 1.14.20.4 | analysis of expression in wheat-rye hybrids |
| 1.14.20.4 | anthocyanin synthase is cloned from Magnolia sprengeri (MsANS) using rapid amplification of complementary DNA ends technology. The full-length MsANS is 1171-bp long and contains a 1080-bp open reading frame encoding a 360 amino acid polypeptide. In a sequence alignment analysis, the deduced MsANS protein showed high identity to ANS proteins from other plants: Prunus salicina var. cordata (74% identity), Ampelopsis grossedentata (74% identity), Pyrus communis (73% identity), and Prunus avium (73% identity) |
| 1.14.20.4 | expressed in Escherichia coli |
| 1.14.20.4 | expressed in Escherichia coli strain DH5alpha |
| 1.14.20.4 | expression in Escherichia coli |
| 1.14.20.4 | expression in Escherichia coli JM109 |
| 1.14.20.4 | expression in Escherichia coli, overexpression in rice mutant Nootripathu, which accumulates proanthocyanidins exclusively in pericarp |
| 1.14.20.4 | expression in Saccharomyces cerevisiae |
