EC Number |
---|
1.1.2.8 | - |
1.1.2.8 | a plasmid construct is used to express PQQ-DH9. DH9. The expression host is a derivative Gluconobacter sp. strain of CHM43, which lacks the genes for PQQ-dependent glycerol dehydrogenase and the membrane-bound alcohol dehydrogenase and consequently has minimal ability to oxidize primary and secondary alcohols. The membranes of the transformant exhibited considerable d-arabitol dehydrogenase activity, whereas the reference strain did not, even if it had PQQ-DH9-encoding genes in the chromosome and harbored the empty vector. This suggests that PQQ-DH9 is not expressed in the genome |
1.1.2.8 | Acetobacter pasteurianus JSTS subunits I (adhA) and II (adhB) of the enzyme (PQQ-ADH) are amplified by PCR, subcloned into a vector, and transformed back into Acetobacter pasteurianus JST-S. The acetic acid production is improved by the engineered strain (61.42 g/l) while the residual ethanol content (4.18 g/l) is decreased |
1.1.2.8 | C-terminally His-tagged wild-type and mutant enzyme forms are expressed in Escherichia coli BL21(DE3) |
1.1.2.8 | gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs |
1.1.2.8 | gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs |
1.1.2.8 | gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs |
1.1.2.8 | gene exaF, or exaA, DNA and amino acid sequence determination and analysis, functional recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain TOP10 |
1.1.2.8 | sequence comparisons and phylogenetic tree, real-time reverse transcriptase PCR expression analysis |