EC Number |
Reference |
---|
1.1.1.26 | cloned and expressed in Escherichia coli |
727414 |
1.1.1.26 | exclusive localization in the cytosol of transgenic Arabidopsis plants co-expressing GFP-GLYR1 and and Cherry-PTS1, a fusion protein consisting of the Cherry fluorescent protein linked to the PTS1 of the peroxisomal enzyme hydroxypyruvate reductase. Expression of N-terminal GFP-tagged or Myc-tagged GLYR1 in tobacco BY-2 cell cytosol. GFP- or Myc-tagged GLYR1 is competent, at least partially, for import into peroxisomes, since replacement of the C-terminal glutamate in GLYR1 with leucine, which yields a canonical PTS1 (i.e., a C-terminal small-basic-hydrophobic tripeptide motif), results in the modified fusion protein (GFPGLYR1-E to L and Myc-GLYR1-E to L) being dual localized to the cytosol and peroxisomes in BY-2 cells |
722899 |
1.1.1.26 | expression in Epicurean coli BL21 (DE3) as fusion protein from a His-tag plasmid |
286417 |
1.1.1.26 | expression in Escherichia coli |
715548 |
1.1.1.26 | expression of His6-tagged enzyme in Escherichia coli srain BL21 |
685901 |
1.1.1.26 | gene gxrA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21 |
685349 |