Cloned (Comment) | Organism |
---|---|
cloned and expressed in Escherichia coli | Thermococcus litoralis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 1 mM, 95% inhibition | Thermococcus litoralis | |
CuCl2 | 1 mM, 66% inhibition | Thermococcus litoralis | |
dithiothreitol | 1 mM, 50% inhibition | Thermococcus litoralis | |
HgCl2 | 1 mM, complete inhibition | Thermococcus litoralis | |
additional information | a significant effect on the activity is not observed with 1 mM EDTA, 1 mM iodoacetate and 1 mM 2-mercaptoethanol | Thermococcus litoralis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.067 | - |
NADH | pH 6.5, 50°C | Thermococcus litoralis | |
0.73 | - |
glyoxylate | pH 6.5, 50°C | Thermococcus litoralis | |
1.3 | - |
Hydroxypyruvate | pH 6.5, 50°C | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36807 | - |
2 * 36807, calculated from sequence | Thermococcus litoralis |
37000 | - |
2 * 37000, SDS-PAGE | Thermococcus litoralis |
76000 | - |
non-denaturimg gradient PAGE | Thermococcus litoralis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | Thermococcus litoralis | - |
glyoxylate + NADH + H+ | - |
? | |
glycolate + NAD+ | Thermococcus litoralis DSM 5473 | - |
glyoxylate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus litoralis | Q9C4M5 | - |
- |
Thermococcus litoralis DSM 5473 | Q9C4M5 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus litoralis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
44.5 | - |
pH 6.5, 50°C | Thermococcus litoralis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycolate + NAD+ | - |
Thermococcus litoralis | glyoxylate + NADH + H+ | - |
? | |
glycolate + NAD+ | - |
Thermococcus litoralis DSM 5473 | glyoxylate + NADH + H+ | - |
? | |
glyoxylate + NADH + H+ | NADPH exhibits 7.5% of the activity compared to NADH | Thermococcus litoralis | glycolate + NAD+ | - |
? | |
glyoxylate + NADH + H+ | NADPH exhibits 7.5% of the activity compared to NADH | Thermococcus litoralis DSM 5473 | glycolate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor | Thermococcus litoralis | D-glycerate + NAD+ | - |
? | |
hydroxypyruvate + NADH + H+ | the relative activity for hydroxypyruvate is one-quarter that of glyoxylate in the presence of NADH as an electron donor | Thermococcus litoralis DSM 5473 | D-glycerate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37000, SDS-PAGE | Thermococcus litoralis |
homodimer | 2 * 36807, calculated from sequence | Thermococcus litoralis |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent glyoxylate reductase | - |
Thermococcus litoralis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Thermococcus litoralis |
90 | - |
- |
Thermococcus litoralis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
10 min, stable up to | Thermococcus litoralis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Thermococcus litoralis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH exhibits rather low activity as an electron donor compared with NADH | Thermococcus litoralis |