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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104gene pdcA, overexpression of His-tagged truncated enzyme, lacking the transmembrane region, in Escherichia coli strain JM109, subcloning in Escherichia coli strain C600
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104gene pgdA, in the wild type, pgdAmRNAhalf-life is 13 min, whereas the half-life for the acnB strain is 7 min, apo-AcnB binds to the 3'-untranslated region of the pgdA RNA transcript. AcnB-pgdA transcript binding is abolished by the addition of iron. Real-time quantitative PCR enzyme expression analysis and RNA footprinting
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104gene Rv1096, sequence comparisons, recombinant expression in Escherichia coli strain ER2566 and in Mycobacterium smegmatis strain mc2155, deacetylation of cell wall peptidoglycan by the recombinant enzyme leads to lysozyme resistance in Mycobacterium smegmatis
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104generation of mutant (pgdA and pgdAadr) and revertant strains
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104the gene bc1960 is cloned and expressed in Escherichia coli
Display the word mapDisplay the reaction diagram Show all sequences 3.5.1.104the gene bc3618 is cloned and expressed in Escherichia coli
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