EC Number   |
|---|
   3.4.21.B12 | expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus |
| 3.4.21.B12 | expression of inactive zymogen in human 293 kidney cells |
| 3.4.21.B12 | gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9 |
| 3.4.21.B12 | into the BamH/HindIII restriction sites of the pQE30 vector and expressed in Escherichia coli M15[pREP4] cells |
| 3.4.21.B12 | KLK4, 5, 6, and 7 full-length cDNA cloned into vector pRc/RSV. OV-MZ-6 ovarian cancer cells stably co-transfected with plasmids expressing K4-7. These OVKLK4+5+6+7 cells inoculated into the peritoneal cavity of nude mice |
| 3.4.21.B12 | KLK4-pIB/V5-His construct expressed in Sf9 cells. PC-3 cell stimulated with KLK4 |
| 3.4.21.B12 | overexpression of KLK4 in PC-3 cells |
