3.4.21.B12	-	
3.4.21.B12	adenovirus-mediated ectopic expression of KLK4 in PC-3 cells and DU145 cells	
3.4.21.B12	construction of a chimeric enzyme with exchange of the pro-piece of the zymogen for that of prostate-specific antigen PSA to create an activation site suceptible for trypsin-type proteases, expression in Escherichia coli strain BL21(DE3)	
3.4.21.B12	DNA and amino acid sequence determination and analysis	
3.4.21.B12	DNA sequence determination and analysis, chromosome mapping and genomic organization	
3.4.21.B12	DNA sequence determination and analysis, chromosome mapping and genomic organization, promotor analysis	
3.4.21.B12	expressed in Escherichia coli	
3.4.21.B12	expressed in Escherichia coli M15(pREP4) cells and in insect cells (nur hk5)	
3.4.21.B12	expressed in Rattus norvegicus and Mus musculus	
3.4.21.B12	expression in Escherichia coli strain AD 494 (DE3) with and without the pro-peptide	
3.4.21.B12	expression in Spodoptera frugiperda SF9 cells via transfection with baculovirus	
3.4.21.B12	expression of inactive zymogen in human 293 kidney cells	
3.4.21.B12	gene KLK4, coupling of the coding sequence of mature enzyme to the pro-domain of prostate specific antigen at the 5' end, and V5 and poly-histidine encoding sequences at the 3' end in plasmid pIBm recombinant expression as inactive zymogen in Spodoptera frugiperda insect cell line Sf9	
3.4.21.B12	into the BamH/HindIII restriction sites of the pQE30 vector and expressed in Escherichia coli M15[pREP4] cells	
3.4.21.B12	KLK4, 5, 6, and 7 full-length cDNA cloned into vector pRc/RSV. OV-MZ-6 ovarian cancer cells stably co-transfected with plasmids expressing K4-7. These OVKLK4+5+6+7 cells inoculated into the peritoneal cavity of nude mice	
3.4.21.B12	KLK4-pIB/V5-His construct expressed in Sf9 cells. PC-3 cell stimulated with KLK4	
3.4.21.B12	overexpression of KLK4 in PC-3 cells