EC Number   |
|---|
   3.2.1.166 | recombinant expression of GST-tagged enzyme from vector pGEX-2TK-heparanase in Escherichia coli strain BL21 |
| 3.2.1.166 | recombinant expression of His-tagged smaller 8 kDa subunit in Escherichia coli strain BL21 (DE3)plysS |
| 3.2.1.166 | recombinant expression of myc-DDK-tagged proheparanase in HEK-293T cells |
| 3.2.1.166 | recombinant expression of the His-tagged enzyme from pET-15b expression vector containing a TEV cleavage site in Escherichia coli BL21-DE3 codon plus |
| 3.2.1.166 | single gene, recombinant expression in Spodoptera frugiperda cells via baculovrius transfection method. The two subunits cotranslationally fold into mature heterodimeric HPSE, bypassing the 65 kDa proenzyme form and ensuring expression of only active enzyme |
| 3.2.1.166 | stably expressed in NS0 myeloma cells |
