3.2.1.166	-	
3.2.1.166	expressed in Mus musculus	
3.2.1.166	expression in CHO cells	
3.2.1.166	expression in COS-7 cells	
3.2.1.166	expression of an N-terminally His-tagged truncated enzyme form, residues 36-543, containing a TEV protease cleavage site, in Escherichia coli strain BL21-CodonPlus (DE3)-RIL	
3.2.1.166	expression of the cloned cDNA in insect and mammalian cells yields 65000 Da and 50000 Da recombinant heparanase proteins. The 50000 Da enzyme represents an N-terminally processed enzyme, at least 100fold more active than the 65000 Da form	
3.2.1.166	expression of the enzyme in Spodoptera frugiperda Sf9 cells and secretion to the cell culture medium	
3.2.1.166	insect cell expression system is used for the generation of large amounts of recombinant protein of high specific activity. Individual subunits are cloned into baculoviral secretory vectors and coexpressed in insect cells	
3.2.1.166	NIH3T3 and COS-7 cells stably transfected with pBK-CMV expression vectors	
3.2.1.166	recombinant expression in insect cells	
3.2.1.166	recombinant expression of GST-tagged enzyme from vector pGEX-2TK-heparanase in Escherichia coli strain BL21	
3.2.1.166	recombinant expression of His-tagged smaller 8 kDa subunit in Escherichia coli strain BL21 (DE3)plysS	
3.2.1.166	recombinant expression of myc-DDK-tagged proheparanase in HEK-293T cells	
3.2.1.166	recombinant expression of the His-tagged enzyme from pET-15b expression vector containing a TEV cleavage site in Escherichia coli BL21-DE3 codon plus	
3.2.1.166	single gene, recombinant expression in Spodoptera frugiperda cells via baculovrius transfection method. The two subunits cotranslationally fold into mature heterodimeric HPSE, bypassing the 65 kDa proenzyme form and ensuring expression of only active enzyme	
3.2.1.166	stably expressed in NS0 myeloma cells