EC Number   |
Reference |
|---|
    2.7.7.60 | gene ispD, recombinant expression of a synthetic gene encoding codon-optimized PvISPD (PVX_081425) with a truncated N-terminal apicoplast localization sequence in Escherichia coli |
760261 |
| 2.7.7.60 | gene ispD, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
762421 |
| 2.7.7.60 | gene ispD, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha |
762421 |
| 2.7.7.60 | gene ispD, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Codon Plus (DE3)-RIL |
760333 |
| 2.7.7.60 | gene ispD, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli |
760261 |
| 2.7.7.60 | gene MCT, DNA and amino acid sequence determination and analysis, recombinant expression of GFP-tagged enzyme in Artemisia annua chloroplasts, recombinant overexpression in Arabidopsis thaliana leading to increases in chlorophyll a and b and carotinoid contents |
762518 |
| 2.7.7.60 | gene PvispD, DNA and amino acid sequence determination and analysis, phylogenetic analysis of ispD proteins, recombinant expression of MBP-tagged PvIspD fusion protein in Escherichia coli strain ER2523 from plasmid pMALc2X-PvispD |
761199 |
| 2.7.7.60 | hyperexpression in Escherichia coli XL1-Blue |
393244 |
| 2.7.7.60 | into the vector pT7Blue for sequencing, a cDNA library is constructed in a PDONR222 vector |
691382 |
| 2.7.7.60 | overexpression of several genes that could complement the defect of the mutants |
393243 |
