2.7.7.60 - 656263, 676369 2.7.7.60 expressed as His-tag fusion protein in Escherichia coli BL21(DE3) 661089 2.7.7.60 expressed as His-tag fusion protein in Escherichia coli M15 660697 2.7.7.60 expressed in Escherichia coli as a His-tagged fusion protein 726274 2.7.7.60 expressed in Escherichia coli BL21(DE3) 393240, 393241 2.7.7.60 expressed in Escherichia coli BL21(DE3)-RP 393239 2.7.7.60 expressed in Escherichia coli M15(pREP4) 393242 2.7.7.60 from genomic DNA in pET28a for expression with N-terminal hexa-His tag in Escherichia coli BL21 (DE3) for 12 h at 16°C 692926 2.7.7.60 gene ispD, located on chromosome 1, DNA and amino acid sequence determination and analysis, phylogenetic analysis 761602 2.7.7.60 gene ispD, recombinant expression od wild-type enzyme in Escherichia coli 760261 2.7.7.60 gene ispD, recombinant expression of a synthetic gene encoding codon-optimized PvISPD (PVX_081425) with a truncated N-terminal apicoplast localization sequence in Escherichia coli 760261 2.7.7.60 gene ispD, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) 762421 2.7.7.60 gene ispD, recombinant expression of GST-tagged enzyme in Escherichia coli strain DH5alpha 762421 2.7.7.60 gene ispD, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Codon Plus (DE3)-RIL 760333 2.7.7.60 gene ispD, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli 760261 2.7.7.60 gene MCT, DNA and amino acid sequence determination and analysis, recombinant expression of GFP-tagged enzyme in Artemisia annua chloroplasts, recombinant overexpression in Arabidopsis thaliana leading to increases in chlorophyll a and b and carotinoid contents 762518 2.7.7.60 gene PvispD, DNA and amino acid sequence determination and analysis, phylogenetic analysis of ispD proteins, recombinant expression of MBP-tagged PvIspD fusion protein in Escherichia coli strain ER2523 from plasmid pMALc2X-PvispD 761199 2.7.7.60 hyperexpression in Escherichia coli XL1-Blue 393244 2.7.7.60 into the vector pT7Blue for sequencing, a cDNA library is constructed in a PDONR222 vector 691382 2.7.7.60 overexpression of several genes that could complement the defect of the mutants 393243