EC Number |
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2.7.1.17 | expression in Escherichia coli BL21(DE3) |
2.7.1.17 | expression in Escherichia coli. Expression of XYL3 using its Pichia stipitis promoter increase Saccharomyces cerevisiae D-xylulose consumption 3fold and enables the transformants to produce ethanol from a mixture of xylulose and xylose, whereas the parental strain only accumulates xylitol. In vitro, D-xylulokinase activity in recombinant Saccharomyces cerevisiae is sixfold higher with a multicopy than with a single-copy XYL3 plasmid, but ethanol production decreases with increased copy number |
2.7.1.17 | gene xyl3, the xylose-metabolic gene XYL3 from Scheffersomyces stipitis is introduced into Saccharomyces cerevisiae |
2.7.1.17 | overexpression in Saccharomyces cerevisiae |
2.7.1.17 | overexpression of the gene on Saccharomyces cerevisiae H158 and CEN-PK also expressing xylose reductase and xylitol dehydrogenase. Effect on fermentation of xylose and lignocellulosic hydrolysate |
2.7.1.17 | overexpression of xylulokinase in Saccharomyces cerevisiae is detrimental to cell growth on xylulose but not on glucose |
2.7.1.17 | recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain H. polymorpha |
2.7.1.17 | recombinant XYL3 gene driven by the HpGAP promotor transformed into the recipient strain Hansenula polymorpha |
2.7.1.17 | use of Pichia stipitis strain FPL-YS30 without D-xylulokinase-activity cause in the deletion of gene xyl3 |
2.7.1.17 | xylB gene expressed in Escherichia coli K12 |