EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
4.6.1.13 | dibutyrylphosphatidylinositol |
is a poor substrate, necessitating long incubation times (2 to 5 h) if the same enzyme concentration is to be used in the absence and presence of salts and amphiphiles |
Listeria monocytogenes |
1D-myo-inositol 1,2-(cyclic)-phosphate + 1,2-dibutyryl-sn-glycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
- |
Oryza sativa |
1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
- |
Bacillus thuringiensis |
1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
- |
Bacillus thuringiensis N168C |
1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
- |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
natural substrate |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
a catalytic diad at the active site composed of Asp-274 and His-32 is involved in substrate-assisted catalysis, its function is to hydrogen-bond with the 2-OH of phosphatidylinositol to form a catalytic triad, catalytic mechanism |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
PI-PLC catalyzes in a second step the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to form myo-inositol 1-phosphate |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
aggregated substrate is preferred over monomeric substrate |
Bacillus thuringiensis |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
a cyclic phosphodiesterase activity of PI-PLC converts 1D-myo-inositol 1,2-cyclic phosphate to inositol 1-phosphate |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
catalytic mechanism, role of Arg-69, the bidentate nature of Arg-69 is the origin of the large thio effects and stereoselectivity in PI-PLC, its function is to bring the phosphate group and the 2-OH group of inositol into proximity and to induce proper alignment for nucleophilic attack, and possibly to lower the pKa of the 2-OH |
Bacillus sp. (in: Bacteria) |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
catalyzes the cleavage of the phosphorus-oxygen bond in phosphatidylinositol, catalytic role of aspartate in a short strong hydrogen bond of the Asp274-His32 catalytic dyad, catalytic mechanism, active site structure |
Bacillus thuringiensis |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |