EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   4.6.1.13 | more |
Bacillus anthracis enzyme down-modulates dendritic cell function und T cell responses, possibly by cleaving GPI-anchored proteins important for TLR-mediated dendritic cell activation |
Bacillus anthracis |
? |
- |
? |
| 4.6.1.13 | more |
the enzyme may have a role in Bacillus anthracis pathogenesis |
Bacillus anthracis |
? |
- |
? |
| 4.6.1.13 | more |
enzyme from Bacillus anthracis unlike the ortholog from Listeria monocytogenes shows high activity on glycosylphosphatidylinositol-anchored proteins |
Bacillus anthracis |
? |
- |
? |
| 4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
- |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
| 4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
natural substrate |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
| 4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
a catalytic diad at the active site composed of Asp-274 and His-32 is involved in substrate-assisted catalysis, its function is to hydrogen-bond with the 2-OH of phosphatidylinositol to form a catalytic triad, catalytic mechanism |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
PI-PLC catalyzes in a second step the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to form myo-inositol 1-phosphate |
? |
| 4.6.1.13 | 1-phosphatidyl-1D-myo-inositol |
cleaves phosphatidylinositol in a rapid intramolecular transphosphorylation reaction forming the products, in a second reaction the cyclic phosphorylase activity of PI-PLC catalyzes the slow hydrolysis of 1D-myo-inositol 1,2-cyclic phosphate to D-myo-inositol 1-phosphate, utilizes His-32 and His-82 in a general acid catalysis mechanism |
Bacillus cereus |
1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol |
- |
? |
| 4.6.1.13 | butyl-fluorescein myo-inositol phosphate |
two substrate molecules bind to enzyme, one at the active site and one at a subsite, causing an increase in activity, subsite interactions of PI-PLC |
Bacillus cereus |
D-myo-inositol 1,2-cyclic phosphate + butyl-fluorescein |
- |
? |
| 4.6.1.13 | lysophosphatidylinositol + H2O |
- |
Bacillus cereus |
? |
- |
? |
| 4.6.1.13 | methyl-fluorescein myo-inositol phosphate |
substrate binds only to the active site and not to the activator site |
Bacillus cereus |
D-myo-inositol 1,2-cyclic phosphate + methyl-fluorescein |
- |
? |
