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3.5.4.35
cytosine8 in tRNA + H2O
all canonical tRNAs have a uridine at position 8, involved in maintaining tRNA tertiary structure. The hyperthermophilic archaeon Methanopyrus kandleri harbors 30 (out of 34) tRNA genes with cytidine at position 8. The enzyme catalyzes C-to-U editing at this location. The presence of this C-to-U editing enzyme guarantees the proper folding and functionality of all Methanopyrus kandleri tRNAs
Methanopyrus kandleri
uracil8 in tRNA + NH3
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3.5.4.35
cytosine8 in tRNA + H2O
the enzyme is specific for C deamination at position 8, requires only the acceptor stem hairpin for activity. CDAT8 is able to recognize 30 different tRNAs mainly by interacting with the tRNA acceptor stem, including the universal 3'-CCA end, and a flexible base C8
Methanopyrus kandleri
uracil8 in tRNA + NH3
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