EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
    3.2.1.68 | starch + H2O |
hydrolysis of alpha-1,6-glycosidic linkages |
Amaranthus cruentus |
? |
- |
? |
| 3.2.1.68 | amylopectin + H2O |
- |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | Arabidopsis starch + H2O |
- |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | beta-limit dextrin + H2O |
- |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | maize beta-limit dextrin + H2O |
- |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | more |
AtISA1 and AtISA2 are required for the production of a functional isoamylase-type of debranching enzyme named Iso1, the major isoamylase found in leaves. The absence of Iso1 leads to an 80% decrease in the starch content and to accumulation of water-soluble polysaccharides whose structure is similar to glycogen |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | more |
AtISA1/AtISA2 isoamylase influences glucan branching pattern |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | more |
glucan debranching occurs primarily at the granule surface via ISA3, but in its absence soluble branched glucans are debranched in the stroma via limit dextrinase. Isoamylase acts at the surface of the starch granule. Atisa3 mutants have more leaf starch and a slower rate of starch breakdown than wild-type plants |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | more |
mutant lines carrying a defect in AtISA3 display a strong starch-excess phenotype at the end of both the light and the dark phases accompanied by a small modification of the amylopectin structure |
Arabidopsis thaliana |
? |
- |
? |
| 3.2.1.68 | more |
synthesis of amylopectin and phytoglycogen by associated and separated ISA1 and ISA2, respectively, overview |
Arabidopsis thaliana |
? |
- |
? |
