EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   2.4.2.12 | ADP + ATP |
isotope exchange reaction (in presence of radiolabeled ADP and ATP but in absence of other reactants, meaning without NMN synthesis) requires high-energy phosphorylated NAMPT |
Homo sapiens |
ATP + ADP |
- |
r |
| 2.4.2.12 | ATP + H2O |
DELTAG° = -7.3, in presence of NAMPT slow steady-state reaction after initial burst, ATPase activity in absence of other substrates, but can be modulated by addition of single substrates and mixtures |
Homo sapiens |
ADP + phosphate |
- |
? |
| 2.4.2.12 | ATP + NAMPT |
autophosphorylation (DELTAG° = 1.9 kcal/mol, Keq = 0.047) is unfavourable and appears as initial burst of ADP generation |
Homo sapiens |
ADP + phospho-NAMPT |
at 2.5 mM ATP and 46 microM NAMPT 77% of NAMPT is phosphorylated (proposed: His247 phospho-NAMPT), hydrolysis of phospho-NAMPT at 0.8/min, active phosphorylated NAMPT species neither isolated nor characterized yet |
r |
| 2.4.2.12 | more |
FK866, a highly specific noncompetitive inhibitor of nicotinamide phosphoribosyltransferase, represents a novel mechanism for induction of tumor cell apoptosis in tissues with a high demand for NAD+ |
Homo sapiens |
? |
- |
? |
| 2.4.2.12 | more |
pathway reconstitution in vitro using recombinant enzymes, overview |
Mus musculus |
? |
- |
? |
| 2.4.2.12 | more |
NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover |
Mus musculus |
? |
- |
? |
| 2.4.2.12 | more |
NMPRTase is a crucial enzyme in the salvage pathway of NAD+ biosynthesis and has important functions in regulating NAD+ levels in cells undergoing substantial NAD+ turnover |
Homo sapiens |
? |
- |
? |
| 2.4.2.12 | more |
rate-limiting enzyme for NAD+ salvage from nicotinamide. Replicative senescence of smooth muscle cells is preceded by a marked decline in the expression and activity of Nampt. Nampt is a longevity protein that can add stress-resistant life to human smooth muscle cells by optimizing SIRT1-mediated p53 degradation |
Homo sapiens |
? |
- |
? |
| 2.4.2.12 | more |
visfatin mimics insulin signaling by binding to the insulin receptor with an affinity similar to that of insulin and does not share the binding site with insulin on the insulin receptor |
Rattus norvegicus |
? |
- |
? |
| 2.4.2.12 | more |
facultative ATP hydrolysis activity, not tightly coupled to NMN synthesis |
Homo sapiens |
? |
- |
? |
