EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
2.4.1.27 | more |
the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview |
Tequatrovirus T4 |
? |
- |
? |
2.4.1.27 | more |
the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview |
Tequatrovirus T4 |
? |
- |
? |
2.4.1.27 | more |
enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme |
Tequatrovirus T4 |
? |
- |
? |
2.4.1.27 | UDP-D-glucose + 5-hydroxymethyl cytosine |
5-hydroxymethyl cytosine residues of duplex DNA |
Tequatrovirus T4 |
UDP + 5-hydroxymethyl cytosine beta-D-glucoside |
- |
? |
2.4.1.27 | UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA |
- |
Tequatrovirus T4 |
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA |
recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme |
? |
2.4.1.27 | UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA |
- |
Tequatrovirus T4 |
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA |
- |
? |
2.4.1.27 | UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues |
- |
Tequatrovirus T4 |
UDP + ? |
- |
? |
2.4.1.27 | UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues |
- |
Tequatrovirus T4 |
UDP + ? |
- |
? |
2.4.1.27 | UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues |
- |
Tequatrovirus T4 |
UDP + ? |
- |
? |
2.4.1.27 | UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues |
- |
Tequatrovirus T4 |
UDP + ? |
- |
? |