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Information on EC 2.4.1.27 - DNA beta-glucosyltransferase
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EC Tree
2.4.1.27 DNA beta-glucosyltransferaseSpecify your search results
Word Map
- 2.4.1.27
- glycosyltransferases
-
bacteriophage
-
glucosylate
-
interdomain
-
base-flipping
- duplex
- tdp
- maltodextrin
-
substrate-free
-
co-crystal
-
dna-modifying
- udp-glcnac
-
5-hydroxymethyl
- vancomycin
- glycosylases
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ligand-bound
-
abasic
-
sialyltransferases
-
flipped-out
-
bidomain
- udp-n-acetylglucosamine
- analysis
- medicine
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
beta-gt, gt-b glycosyltransferase, t4 beta-glucosyltransferase, t4 phage beta-glucosyltransferase, dna beta-glucosyltransferase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
BGT
glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate beta-
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UDP glucose-DNA beta-glucosyltransferase
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-
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uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase
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additional information
Tequatrovirus T4
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the enzyme belongs to the GT-B glycosyltransferase family
BGT
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
catalytic mechanism
Tequatrovirus T4
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
base-flipping mechanism
Tequatrovirus T4
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
catalytic mechanism, substrate binding structures, overview
Tequatrovirus T4
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA = UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hexosyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
UDP-glucose:DNA beta-D-glucosyltransferase
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CAS REGISTRY NUMBER
COMMENTARY
9030-14-2
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA
Tequatrovirus T4
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-
-
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UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
Tequatrovirus T4
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5-hydroxymethyl cytosine residues of duplex DNA
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-
?
UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
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recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme
-
?
UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA
Tequatrovirus T4
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-
-
-
?
UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
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-
-
-
?
UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
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-
-
-
?
UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
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-
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?
UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues
UDP + ?
Tequatrovirus T4
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-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
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-
-
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
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-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
DNA-modifying enzyme
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-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme forms part of a phage DNA protection system
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
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the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
the enzyme modifies T4-DNA
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-
?
additional information
?
-
Tequatrovirus T4
-
the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
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?
additional information
?
-
Tequatrovirus T4
-
the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview
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-
?
additional information
?
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Tequatrovirus T4
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enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-alpha-D-glucose + 5-hydroxymethylcytosine-containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine-containing DNA
Tequatrovirus T4
-
-
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UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
Tequatrovirus T4
-
5-hydroxymethyl cytosine residues of duplex DNA
-
-
?
additional information
?
-
Tequatrovirus T4
-
the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
-
-
-
-
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
DNA-modifying enzyme
-
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme forms part of a phage DNA protection system
-
?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
Tequatrovirus T4
-
the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
-
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mg2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mn2+
Tequatrovirus T4
-
four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
competitive inhibitor with respect to 5-hydroxymethyl cytosine containing DNA and mixed inhibitor with respect to UDP-glucose
UDP
Tequatrovirus T4
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competitive inhibitor with respect to UDP-glucose and mixed inhibitor with respect to 5-hydroxymethyl cytosine containing DNA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00023
DNA containing 12 5-hydroxymethyl cytosine residues
Tequatrovirus T4
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pH 7.9, 25°C
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0.00022
DNA containing 24 5-hydroxymethyl cytosine residues
Tequatrovirus T4
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pH 7.9, 25°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.003
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
substrate UDP-glucose, pH 7.9, 25°C
0.004
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
Tequatrovirus T4
-
substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C
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IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Tequatrovirus T4
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). GSTB_BPT4
351
0
40666
Swiss-Prot
other Location (Reliability: 2)
A0A8K1S473_9CAUD
358
0
41781
TrEMBL
other Location (Reliability: 2)
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
Tequatrovirus T4
-
structural analysis of domain folding, substrate binding, catalytic mechanism, and base flipping mechanism, overview
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the recombinant enzyme in the presence and absence of the substrate, UDPglucose
Tequatrovirus T4
-
crystallization of the enzyme with UDPglucose and a 13mer DNA fragment containing an abasic site. Two crystal structures are obtained of a ternary complex BGT-UDP-DNA at 1.8 A and 2.5 A resolution, one with a Tris molecule and the other with a metal ion at the active site
Tequatrovirus T4
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Tequatrovirus T4
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
analysis
Tequatrovirus T4
-
use of recombinant enzyme to estimate global 5-hydroxymethyl cytosine content in a variety of genomic DNAs. Most of the genomic DNAs derived from vertebrate tissue and cell lines contain 5-hydroxymethyl cytosine. DNA from mouse, human, and bovine brains displays 0.5?0.9% of the total nucleotides as 5-hydroxymethyl cytosine, which is higher compared to the levels found in other tissues. A comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
medicine
Tequatrovirus T4
-
a comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Freemont, P.S.; Ruger, W.
Crystallization and preliminary X-ray studies of T4 phage beta-glucosyltransferase
J. Mol. Biol.
203
525-526
1988
Tequatrovirus T4
brenda
Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Rueger, W.; Morera, S.
Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Acta Crystallogr. Sect. D
58
1484-1486
2002
Tequatrovirus T4 (P04547)
brenda
Morera, S.; Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Freemont, P.S.; Janin, J.; Ruger, W.
High resolution crystal structures of T4 phage beta-glucosyltransferase: Induced fit and effect of substrate and metal binding
J. Mol. Biol.
311
569-577
2001
Tequatrovirus T4
brenda
Vrielink, A.; Rueger, W.; Driessen, H.P.C.; Freemont, P.S.
Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
EMBO J.
13
3413-3422
1994
Tequatrovirus T4
brenda
Morera, S.; Imberty, A.; Aschke-Sonnenborn, U.; Ruger, W.; Freemont, P.S.
T4 phage beta-glucosyltransferase: Substrate binding and proposed catalytic mechanism
J. Mol. Biol.
292
717-730
1999
Tequatrovirus T4
brenda
Lariviere, L.; Morera, S.
A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog
J. Mol. Biol.
324
483-490
2002
Tequatrovirus T4 (P04547)
brenda
Artymiuk, P.J.; Rice, D.W.; Poirrette, A.R.; Willett, P.
beta-Glucosyltransferase and phosphorylase reveal their common theme
Nat. Struct. Biol.
2
117-120
1995
Tequatrovirus T4
brenda
Lariviere, L.; Gueguen-Chaignon, V.; Morera, S.:
Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: Glucose binding and identification of the catalytic base for a direct displacement mechanism
J. Mol. Biol.
330
1077-1086
2003
Tequatrovirus T4 (P04547)
brenda
Lariviere, L.; Sommer, N.; Morera, S.
Structural evidence of a passive base-flipping mechanism for AGT, an unusual GT-B glycosyltransferase
J. Mol. Biol.
352
139-150
2005
Tequatrovirus T4
brenda
Terragni, J.; Bitinaite, J.; Zheng, Y.; Pradhan, S.
Biochemical characterization of recombinant alpha-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes
Biochemistry
51
1009-1019
2012
Tequatrovirus T4
brenda
Yin, H.; Yang, Z.; Wang, H.; Zhou, Y.; Ai, S.
Electrochemical biosensor for hydroxymethylated DNA detection and beta-glucosyltransferase activity assay based on enzymatic catalysis triggering signal amplification
Sens. Actuators B Chem.
243
602-608
2017
Tequatrovirus T4
-
brenda
Jiang, W.; Yin, H.; Zhou, Y.; Duan, J.; Li, H.; Wang, M.; Waterhouse, G.; Ai, S.
A novel electrochemiluminescence biosensor for the detection of 5-methylcytosine, TET 1 protein and beta-glucosyltransferase activities based on gold nanoclusters-H2O2 system
Sens. Actuators B Chem.
274
144-151
2018
Tequatrovirus T4
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brenda
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