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Information on EC 2.4.1.27 - DNA beta-glucosyltransferase for references in articles please use BRENDA:EC2.4.1.27Word Map on EC 2.4.1.27
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The expected taxonomic range for this enzyme is: Escherichia virus T4
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DNA beta-glucosyltransferase
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Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA
Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA
catalytic mechanism
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Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA
base-flipping mechanism
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Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA
catalytic mechanism, substrate binding structures, overview
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Transfers a beta-D-glucosyl residue from UDP-glucose to an hydroxymethylcytosine residue in DNA
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hexosyl group transfer
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UDP-glucose:DNA beta-D-glucosyltransferase
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glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate beta-
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GT-B glycosyltransferase
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T4 phage beta-glucosyltransferase
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T4-beta-glucosyl transferase
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T4-HMC-beta-glucosyl transferase
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UDP glucose-DNA beta-glucosyltransferase
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uridine diphosphoglucose-deoxyribonucleate beta-glucosyltransferase
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additional information
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the enzyme belongs to the GT-B glycosyltransferase family
BGT
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Uniprot
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from Escherichia coli
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
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5-hydroxymethyl cytosine residues of duplex DNA
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?
UDP-D-glucose + 5-hydroxymethyl cytosine containing DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing DNA
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recombinant enzyme glucosylates 5-hydroxymethyl cytosine containing DNA in a nonprocessive manner, and binding to either 5-hydroxymethyl cytosine containing DNA or uridine diphosphoglucose substrates is random, with both binary complexes being catalytically competent. The numbers of 5-hydroxymethyl cytosine on target sequences influence the turnover numbers for the recombinant enzyme
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UDP-D-glucose + 5-hydroxymethyl cytosine containing phage T4 DNA
UDP + 5-hydroxymethyl cytosine beta-D-glucoside containing phage T4 DNA
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UDP-D-glucose + DNA containing 12 5-hydroxymethyl cytosine residues
UDP + ?
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UDP-D-glucose + DNA containing 2 5-hydroxymethyl cytosine residues
UDP + ?
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?
UDP-D-glucose + DNA containing 24 5-hydroxymethyl cytosine residues
UDP + ?
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UDP-D-glucose + DNA containing 6 5-hydroxymethyl cytosine residues
UDP + ?
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additional information
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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?
UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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DNA-modifying enzyme
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme forms part of a phage DNA protection system
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme modifies T4-DNA
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additional information
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the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
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additional information
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the alpha-linkage forming enzyme AGT is also able to adopt the fold of a GT-B glycosyltransferase keeping the sugar donor binding mechanism typical for GT-B glycosyltransferases, but showing a unique sugar acceptor binding mode, both substrates are bound to the C-terminal domain, detailed substrate binding structures, catalytic mechanism of both enzyme types, overview
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additional information
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enzyme shows strong preference for the glucosylation of DNA containing symmetrical 5-hydroxymethyl cytosine resiudes over hemi-5-hydroxymethyl cytosine. The enzyme is capable of saturation glucosylation on all conformations of 5-hydroxymethyl cytosine.The numbers of 5-hydroxymethyl cytosine residues on DNA can influence catalysis, especially the rate of catalysis, and hemihydroxymethylated DNA is not the preferred substrate of the enzyme
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
UDP-D-glucose + 5-hydroxymethyl cytosine
UDP + 5-hydroxymethyl cytosine beta-D-glucoside
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5-hydroxymethyl cytosine residues of duplex DNA
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additional information
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the AGT with GT-B glycosyltransferase fold is responsible for DNA base flipping and substrate recognition, mechanism, overview
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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DNA-modifying enzyme
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme forms part of a phage DNA protection system
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme is part of a system by which T-even bacteriophages glycosylate their genomic DNA in order to protect it against nuclease attack
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UDP-alpha-D-glucose + 5-hydroxymethylcytosine containing DNA
UDP + beta-D-glucosyl-5-hydroxymethylcytosine containing DNA
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the enzyme modifies T4-DNA
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Ca2+
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four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mg2+
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four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
Mn2+
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four of the six X-ray structures of the enzyme contain metal ions which activate the enzyme, including Mg2+ in forms 1 and 2 and Mn2+ or Ca2+. Mg2+, Mn2+ and Ca2+ bind near the beta-phosphate of the nucleotide, but they occupy slightly different positions
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5-hydroxymethyl cytosine beta-D-glucoside containing DNA
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competitive inhibitor with respect to 5-hydroxymethyl cytosine containing DNA and mixed inhibitor with respect to UDP-glucose
UDP
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competitive inhibitor with respect to UDP-glucose and mixed inhibitor with respect to 5-hydroxymethyl cytosine containing DNA
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0.0004
5-hydroxymethyl cytosine containing phage T4 DNA
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pH 7.9, 25°C
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0.00023
DNA containing 12 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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0.003
DNA containing 2 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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0.00022
DNA containing 24 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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0.0006
DNA containing 6 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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0.016
UDP-D-glucose
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pH 7.9, 25°C
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1.283
5-hydroxymethyl cytosine containing phage T4 DNA
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pH 7.9, 25°C
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1.033
DNA containing 12 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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2.8
DNA containing 2 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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9.66
DNA containing 24 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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1.666
DNA containing 6 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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3200
5-hydroxymethyl cytosine containing phage T4 DNA
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pH 7.9, 25°C
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4483
DNA containing 12 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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933
DNA containing 2 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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4400
DNA containing 24 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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2766
DNA containing 6 5-hydroxymethyl cytosine residues
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pH 7.9, 25°C
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0.003 - 0.004
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
0.003
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
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substrate UDP-glucose, pH 7.9, 25°C
0.004
5-hydroxymethyl cytosine beta-D-glucoside containing DNA
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substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C
0.002
UDP
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substrate 5-hydroxymethyl cytosine containing DNA, pH 7.9, 25°C
0.009
UDP
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substrate UDP-glucose, pH 7.9, 25°C
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P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
P04547
Enterobacteria phage T4;
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additional information
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structural analysis of domain folding, substrate binding, catalytic mechanism, and base flipping mechanism, overview
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crystal structure of the recombinant enzyme in the presence and absence of the substrate, UDPglucose; ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
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crystallization of the enzyme with UDPglucose and a 13mer DNA fragment containing an abasic site. Two crystal structures are obtained of a ternary complex BGT-UDP-DNA at 1.8 A and 2.5 A resolution, one with a Tris molecule and the other with a metal ion at the active site; ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
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ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
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expression in Escherichia coli
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analysis
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use of recombinant enzyme to estimate global 5-hydroxymethyl cytosine content in a variety of genomic DNAs. Most of the genomic DNAs derived from vertebrate tissue and cell lines contain 5-hydroxymethyl cytosine. DNA from mouse, human, and bovine brains displays 0.5?0.9% of the total nucleotides as 5-hydroxymethyl cytosine, which is higher compared to the levels found in other tissues. A comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
medicine
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a comparison between cancer and healthy tissue genomes suggests a lower percentage of 5-hydroxymethyl cytosine in cancer
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GSTB_BPT4
351
40666
Swiss-Prot
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Freemont, P.S.; Ruger, W.
Crystallization and preliminary X-ray studies of T4 phage beta-glucosyltransferase
J. Mol. Biol.
203
525-526
1988
Escherichia virus T4
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Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Rueger, W.; Morera, S.
Crystallization and preliminary crystallographic study of a ternary complex between the T4 phage beta-glucosyltransferase, uridine diphosphoglucose and a DNA fragment containing an abasic site
Acta Crystallogr. Sect. D
58
1484-1486
2002
Escherichia virus T4 (P04547)
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Morera, S.; Lariviere, L.; Kurzeck, J.; Aschke-Sonnenborn, U.; Freemont, P.S.; Janin, J.; Ruger, W.
High resolution crystal structures of T4 phage beta-glucosyltransferase: Induced fit and effect of substrate and metal binding
J. Mol. Biol.
311
569-577
2001
Escherichia virus T4
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Vrielink, A.; Rueger, W.; Driessen, H.P.C.; Freemont, P.S.
Crystal structure of the DNA modifying enzyme beta-glucosyltransferase in the presence and absence of the substrate uridine diphosphoglucose
EMBO J.
13
3413-3422
1994
Escherichia virus T4
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Morera, S.; Imberty, A.; Aschke-Sonnenborn, U.; Ruger, W.; Freemont, P.S.
T4 phage beta-glucosyltransferase: Substrate binding and proposed catalytic mechanism
J. Mol. Biol.
292
717-730
1999
Escherichia virus T4
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Lariviere, L.; Morera, S.
A base-flipping mechanism for the T4 phage beta-glucosyltransferase and identification of a transition-state analog
J. Mol. Biol.
324
483-490
2002
Escherichia virus T4 (P04547)
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Artymiuk, P.J.; Rice, D.W.; Poirrette, A.R.; Willett, P.
beta-Glucosyltransferase and phosphorylase reveal their common theme
Nat. Struct. Biol.
2
117-120
1995
Escherichia virus T4
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Lariviere, L.; Gueguen-Chaignon, V.; Morera, S.:
Crystal structures of the T4 phage beta-glucosyltransferase and the D100A mutant in complex with UDP-glucose: Glucose binding and identification of the catalytic base for a direct displacement mechanism
J. Mol. Biol.
330
1077-1086
2003
Escherichia virus T4 (P04547)
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Lariviere, L.; Sommer, N.; Morera, S.
Structural evidence of a passive base-flipping mechanism for AGT, an unusual GT-B glycosyltransferase
J. Mol. Biol.
352
139-150
2005
Escherichia virus T4
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Terragni, J.; Bitinaite, J.; Zheng, Y.; Pradhan, S.
Biochemical characterization of recombinant alpha-glucosyltransferase and analysis of global 5-hydroxymethylcytosine in unique genomes
Biochemistry
51
1009-1019
2012
Escherichia virus T4
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