EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.3.99.30 | 1-hydroxylycopene + acceptor |
- |
Neurospora crassa |
1-hydroxy-3',4'-didehydrolycopene + reduced acceptor |
- |
? |
1.3.99.30 | 1-hydroxyneurosporene + acceptor |
- |
Neurospora crassa |
1-hydroxylycopene + reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
- |
Neurospora crassa |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
- |
Phaffia rhodozyma |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products |
Phaffia rhodozyma |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
the enzyme is involved in the carotenoid biosynthesis pathway to beta-carotene and torulene |
Neurospora crassa |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
- |
Phaffia rhodozyma CBS 6938 |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + 5 acceptor |
a decisive reaction for the formation of monocyclic or bicyclic products is the desaturation sequence to lycopene and further on to 3,4-didehydrolycopene. In the nontransformed strain, cyclization of lycopene, which directs the metabolic flux towards astaxanthin, is the dominating reaction. When the gene encoding phytoene desaturase is overexpressed, the five-step desaturation to 3,4-didehydrolycopene is intensified, resulting in an accumulation of torulene and 3-hydroxy-3'-4'-didehydro-beta-psi-caroten-4-one as subsequent products |
Phaffia rhodozyma CBS 6938 |
all-trans-3,4-didehydrolycopene + 5 reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + acceptor |
- |
Neurospora crassa |
all-trans-phytofluene + reduced acceptor |
- |
? |
1.3.99.30 | 15-cis-phytoene + acceptor |
- |
Blakeslea trispora |
all-trans-phytofluene + reduced acceptor |
- |
? |