EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   1.1.1.30 | (R)-3-hydroxybutyrate + NAD+ |
- |
Acidovorax sp. |
acetoacetate + NADH + H+ |
- |
r |
| 1.1.1.30 | (R)-3-hydroxybutyrate + NAD+ |
strain SA1 can degrade poly((R)-3-hydroxybutyrate), i.e. PHB |
Acidovorax sp. |
acetoacetate + NADH + H+ |
- |
r |
| 1.1.1.30 | (R)-3-hydroxybutanoate + NAD+ |
- |
Alcaligenes faecalis |
acetoacetate + NADH |
- |
r |
| 1.1.1.30 | (R)-3-hydroxybutanoate + NAD+ |
- |
Alcaligenes faecalis |
acetoacetate + NADH + H+ |
- |
r |
| 1.1.1.30 | acetoacetate + NADH + H+ |
- |
Alcaligenes faecalis |
(R)-3-hydroxybutanoate + NAD+ |
- |
? |
| 1.1.1.30 | D-3-hydroxybutyrate + NAD+ |
the reversible reactions occur by shuttle movements of a hydrogen negative ion from the C3 atom of the substrate to the C4 atom of NAD+ and from the C4 atom of NADH to the C3 atom of the product. The reaction may be further coupled to the withdrawal of a proton from the hydroxyl group of the substrate by the ionized Tyr155 residue |
Alcaligenes faecalis |
acetoacetate + NADH + H+ |
- |
r |
| 1.1.1.30 | levulinic acid + NADH + H+ |
only the recombinant wild type enzyme with N-terminal His-tag shows activity with levulinic acid |
Alcaligenes faecalis |
4-hydroxyvaleric acid + NAD+ |
- |
? |
| 1.1.1.30 | levulinic acid + NADH + H+ |
the wild type enzyme shows low activity with levulinic acid, while it's a good substrate for mutant enzyme H144L/W187F |
Alcaligenes faecalis |
4-hydroxyvaleric acid + NAD+ |
- |
? |
| 1.1.1.30 | more |
no activity of the wild-type enzyme with levulinic acid, enzyme mutant H144L/W187F is active with levulinic acid producing 4-hydroxyvaleric acid |
Alcaligenes faecalis |
? |
- |
? |
| 1.1.1.30 | (R)-3-hydroxybutanoate + NAD+ |
- |
Bacillus cereus |
acetoacetate + NADH |
- |
r |
