EC Number   |
Substrates |
Organism |
Products |
Reversibility |
|---|
   3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
- |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
cytosine deamination occurs preferentially in CpC (5'GpG/CpC5') |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
in vitro, the enzyme has a preferred sequence motif of T/CCC and shows a 3'->5' like processivity |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
native enzyme demonstrates a preference for deamination of the C residue proximal to the 5'-ssDNA end in the 5'CCC motif and deaminates the two C residues processively |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
phage M13mp2 circular DNA containing a series of in-frame 5'-AAACCCAAA hot motifs embedded in lacZalpha reporter sequence located within a single-stranded gapped region of M13 double-stranded DNA. The third C in the 5'-AAACCCAAA motif is deaminated predominantly |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
the enzyme carries out processive cytosine deamination by randomly binding, sliding and jumping bidirectionally on single-stranded DNA. The deamination by the enzyme proceeds predominantly 3'->5', resulting in preferential deamination at the target located closer to the 5' end of substrate. The enzyme favors deamination at the 5'C residue in the hot-spot motif CCC |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
the enzyme strongly prefers cytosines in a run of C's usually targeting the last base in the run. The enzyme readily converts the third cytosine in CCC to uracil but does not convert the first or the second cytosine in the sequence at detectable levels |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
the enzyme targets 5'-CCCA-3' sequences with 5'-AAACCCAAA-3' recognized most efficiently |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
the full-length enzyme processively deaminates cytidine in two 5'-CCC-3' motifs located on a single-stranded DNA substrate, during one binding event. The full-length enzyme also exerts a 3' to 5' deamination bias by preferentially deaminating the cytidine in the CCC motif near the 5'end of the single-stranded DNA substrate |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
| 3.5.4.B9 | cytosine in single-stranded viral DNA + H2O |
minus strand deamination occurs preferentially at a CCCA sequence. The third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, so the deamination is carried out in a strict 3'-5' order |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
