EC Number   |
Storage Stability |
|---|
   2.7.1.107 | -20°C, in presence of 1 mM dithiothreitol, microsomal and soluble enzyme stable for about 1 week |
| 2.7.1.107 | -80°C, purified isoform DGKepsilon and DGKepsilonDELTA40, 14 weeks, no significant loss of activity |
| 2.7.1.107 | 4°C, -20°C or -70°C, more than 50% of the activity is lost after overnight storage in presence of 10 or 20% glycerol |
| 2.7.1.107 | 4°C, enzyme concentrated by polyethyleneglycol 6000, stable for 1 week |
| 2.7.1.107 | 4°C, purified enzyme in 20 mM Tris-HCl, pH 7.4 with 200 mM NaCl, 3 mM CaCl2, 3 mM MgCl2, 0.5 mM dithiothreitol, and 5% (v/v) glycerol, at least 3 months, no loss of activity |
| 2.7.1.107 | 4°C, purified enzyme, 50% loss of activity after 3 days |
| 2.7.1.107 | the 150000 Da enzyme is very unstable but can be stored at -80°C in presence of bovine serum albumin |
