EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   5.1.3.8 | -999 |
- |
- |
2427 |
| 5.1.3.8 | -999 |
- |
Escherichia coli cells harboring the recombinant plasmid are grown at 37°C in TB medium and induced by adding lactose to a final concentration of 1% lactose, samples are taken every 2 h, and the highest activity is 0.825 U/ml after fermentation for 22 h, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) |
701855 |
| 5.1.3.8 | 0.07818 |
- |
AGE, immobilized recombinant enzyme, the activity yield of immobilized enzyme is approximately 30% of the free enzyme, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) |
701855 |
| 5.1.3.8 | 0.49 |
- |
specific activity in crude extract after expression in Escherichia coli cells, activity is determined in 1 ml of reaction solution (100 mM Tris-HCl, pH 7.5, 100 mM GlcNAc, 10 mM MgCl2, and 2.5 mM ATP) |
701855 |
| 5.1.3.8 | 5.3 |
- |
- |
2428, 2429 |
| 5.1.3.8 | 5.6 |
- |
E242Q, reverse conversion in the presence of 1 mM ATP |
675440 |
| 5.1.3.8 | 6.2 |
- |
R375A, reverse conversion in the presence of 1 mM ATP |
675440 |
| 5.1.3.8 | 6.6 |
- |
E218A, reverse conversion in the presence of 1 mM ATP |
675440 |
| 5.1.3.8 | 8.1 |
- |
E242Q, in the presence of 1 mM ATP |
675440 |
| 5.1.3.8 | 8.9 |
- |
R375K, reverse conversion in the presence of 1 mM ATP |
675440 |
