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Literature summary for 5.1.3.8 extracted from

  • Lee, Y.C.; Wu, H.M.; Chang, Y.N.; Wang, W.C.; Hsu, W.H.
    The central cavity from the (alpha/alpha)6 barrel structure of Anabaena sp. CH1 N-acetyl-D-glucosamine 2-epimerase contains two key histidine residues for reversible conversion (2007), J. Mol. Biol., 367, 895-908.
    View publication on PubMed
Search for more literature for 5.1.3.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Anabaena sp. CH1

Crystallization (Commentary)

Crystallization (Comment) Organism
of its apoform Anabaena sp. CH1

Protein Variants

Protein Variants Comment Organism
C370A site directed mutagenesis Anabaena sp. CH1
E218A site directed mutagenesis Anabaena sp. CH1
E218D site directed mutagenesis Anabaena sp. CH1
E242Q site directed mutagenesis Anabaena sp. CH1
E308A site directed mutagenesis Anabaena sp. CH1
E308D site directed mutagenesis Anabaena sp. CH1
H239A site directed mutagenesis Anabaena sp. CH1
H239N site directed mutagenesis Anabaena sp. CH1
H372A site directed mutagenesis Anabaena sp. CH1
H372I site directed mutagenesis Anabaena sp. CH1
H372N site directed mutagenesis Anabaena sp. CH1
N179A site directed mutagenesis Anabaena sp. CH1
R375A site directed mutagenesis Anabaena sp. CH1
R375K site directed mutagenesis Anabaena sp. CH1
R57A site directed mutagenesis Anabaena sp. CH1
R57K site directed mutagenesis Anabaena sp. CH1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.2
-
 N-acetyl-D-glucosamine E242Q, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
7.4
-
 N-acetyl-D-glucosamine R375K, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
9.2
-
 N-acetyl-D-mannosamine R375K, in the presence of 1 mM ATP Anabaena sp. CH1
13.4
-
 N-acetyl-D-glucosamine E218D, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
13.9
-
 N-acetyl-D-mannosamine R375A, in the presence of 1 mM ATP Anabaena sp. CH1
14.8
-
 N-acetyl-D-mannosamine wild type, at pH 7, in the presence of 1 mM ATP Anabaena sp. CH1
15.5
-
 N-acetyl-D-mannosamine E218A, in the presence of 1 mM ATP Anabaena sp. CH1
15.6
-
 N-acetyl-D-mannosamine wild type, at pH 8, in the presence of 1 mM ATP Anabaena sp. CH1
16.7
-
 N-acetyl-D-glucosamine C370A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
16.7
-
 N-acetyl-D-glucosamine R375A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
21
-
 N-acetyl-D-mannosamine E218D, in the presence of 1 mM ATP Anabaena sp. CH1
21.3
-
 N-acetyl-D-mannosamine C370A, in the presence of 1 mM ATP Anabaena sp. CH1
24.4
-
 N-acetyl-D-glucosamine E218A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
25.4
-
 N-acetyl-D-glucosamine wild type, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
27.2
-
 N-acetyl-D-mannosamine N179A, in the presence of 1 mM ATP Anabaena sp. CH1
32.7
-
 N-acetyl-D-mannosamine wild type, at pH 9, in the presence of 1 mM ATP Anabaena sp. CH1
45.7
-
 N-acetyl-D-glucosamine N179A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
53.8
-
 N-acetyl-D-mannosamine E242Q, in the presence of 1 mM ATP Anabaena sp. CH1
57.33
-
 N-acetyl-D-mannosamine wild type, at pH 6 in the presence of 1 mM ATP Anabaena sp. CH1

Organism

Organism UniProt Comment Textmining
Anabaena sp. CH1 A4UA16
-
-

Purification (Commentary)

Purification (Comment) Organism
of the recombinant proteins, wild type and mutants by immobilized nickel-ion chromatography Anabaena sp. CH1

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
 E242Q, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
6.2
-
 R375A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
6.6
-
 E218A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
8.1
-
 E242Q, in the presence of 1 mM ATP Anabaena sp. CH1
8.9
-
 R375K, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
22.8
-
 E218A, in the presence of 1 mM ATP Anabaena sp. CH1
46.5
-
 E218D, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
50.8
-
 N179A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
54.8
-
 R375A, in the presence of 1 mM ATP Anabaena sp. CH1
85.9
-
 C370A, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
94.3
-
 R375K, in the presence of 1 mM ATP Anabaena sp. CH1
123.8
-
 wild type, reverse conversion in the presence of 1 mM ATP Anabaena sp. CH1
256.4
-
 E218D, in the presence of 1 mM ATP Anabaena sp. CH1
258.5
-
 N179A, in the presence of 1 mM ATP Anabaena sp. CH1
351
-
 C370A, in the presence of 1 mM ATP Anabaena sp. CH1
525.8
-
 wild type, in the presence of 1 mM ATP Anabaena sp. CH1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine
-
 Anabaena sp. CH1 N-acetyl-D-glucosamine
-
 r

Synonyms

Synonyms Comment Organism
bGlcNAc 2-epimerase recombinant protein Anabaena sp. CH1
GlcNAc 2-epimerase
-
 Anabaena sp. CH1
N-acyl-D-glucosamine 2-epimerase
-
 Anabaena sp. CH1
N-acylglucosamine 2-epimerase
-
 Anabaena sp. CH1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Anabaena sp. CH1

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5
-
 Anabaena sp. CH1