EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.1.26.4 | -999 |
- |
- |
134367, 134370, 134371, 134374, 134380, 134387, 134390, 134392, 134397, 134398, 134401, 134406 |
3.1.26.4 | -999 |
- |
activity of wild-type and mutant enzymes at different temperatures |
654569 |
3.1.26.4 | -999 |
- |
activity of wild-type and mutant enzymes, determination by spectrophotometric assay, at different temperatures |
657218 |
3.1.26.4 | -999 |
- |
determination of RNA levels |
655447 |
3.1.26.4 | -999 |
- |
development and validation of a CpRNase HII-based method for activity assay and detection: DNA-rN1-DNA fragments are modified with a fluorophore at the 5'-end and a quencher at the 3'-end to generate molecular beacons, which hybridize with single-stranded DNA to be cleaved by CpRNase HII, the method is suitable for large-scale genotyping, overview |
682205 |
3.1.26.4 | -999 |
- |
reaction velocity at oxidizing and reducing conditions |
656122 |
3.1.26.4 | -999 |
- |
the activity of the recombinant soluble enzyme is slightly higher than that of the recombinant refolded enzyme |
655904 |
3.1.26.4 | 0.000038 |
- |
- |
134080 |
3.1.26.4 | 0.001 |
- |
below, mutant D97A, with 50 mM Mg2+ or 10 mM Mn2+ |
666070 |
3.1.26.4 | 0.0046 |
- |
mutant E98A, with 10 mM Mn2+ |
666070 |