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Literature summary for 3.1.26.4 extracted from
- Human RNase H1 activity is regulated by a unique redox switch formed between adjacent cysteines (2003), J. Biol. Chem., 278, 14906-14912.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Homo sapiens |  |
| DTT | - |
Homo sapiens | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C147A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
| C147A/C148A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
| C148A | site-directed mutagenesis, mutant is active under reducing and oxidizing conditions | Homo sapiens |
| C191A | site-directed mutagenesis, mutant is active under reducing conditions, but shows poor activity under oxidizing conditions | Homo sapiens |
| additional information | construction of deletion mutant H1[DELTA1-73] | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-ethylmaleimide | inhibits wild-type enzyme and deletion mutant H1[DELTA1-73] | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
| Mn2+ | - |
Homo sapiens | |
| additional information | enzyme requires divalent cations | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Homo sapiens | the enzyme is regulated by a unique redox switch formed by adjacent Cys147 and Cys148 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
type 1 enzyme | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Escherichia coli BL21(DE3) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
reaction velocity at oxidizing and reducing conditions | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is regulated by a unique redox switch formed by adjacent Cys147 and Cys148 | Homo sapiens | ? | - |
? | |
| additional information | formation of a disulfide bond, under oxidizing conditions, between Cys147 and Cys148 results in an inactive enzyme conformation | Homo sapiens | ? | - |
? | |
| RNA*DNA hybrid + H2O | enzyme is active only under reducing conditions, wild-type enzyme and deletion mutant H1[DELTA1-73] are inactive under oxidizing conditions | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| RNase H1 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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