EC Number   |
Reference |
|---|
    3.1.1.3 | after unfolding cutinase at 80°C, cooling at 20°C restores the initial conformation |
748394 |
| 3.1.1.3 | insoluble recombinant enzyme is solubilized by 8 M urea, purified and refolded by removing urea in presence of Ca2+ |
652764 |
| 3.1.1.3 | recombinant enzyme from Escherichia coli inclusion bodies by 20 mM Tris-HCl buffer, pH 8.0, and 5.3 M urea, followed by a 100fold dilution |
679716 |
| 3.1.1.3 | recombinant enzyme in inclusion bodies is solubilized by 8 M urea and refolded by removing urea in presence of Ca2+ |
650306 |
| 3.1.1.3 | recombinant LipB68 from Escherichia coli inclusion bodies by 8 M urea and subsequent dialysis, 25 mM Tris-HCl, pH 8.0 |
677737 |
| 3.1.1.3 | recombinant wild-type LipA and LipH mutant 1H8 from Escherichia coli strain BL21(DE3) inclusion bodies, denaturation by treatment for 1 h at 37°C with 100 mM Tris-HCl, pH 8.0, 8 M urea, followed by in vitro refolding to enzymatic activity performed for 3 h by adding equimolar ratios of LipH and sudden 10fold dilution in a buffer containing 50 mM Tris-HCl, pH 8.0, 3.5 mM CaCl2, 0.7 mM laurylmaltoside and 45% v/v glycerol |
692387 |
| 3.1.1.3 | refolding of wild-type LipA from inclusion bodies solublized by 8 M urea, refolded with a 56-amino acid truncated chaperone DELTALipBHis6 at 4°C for 24 h in water in a ratio of 1:1 |
666825 |
| 3.1.1.3 | solubilized recombinant enzyme from inclusion bodies after expression in Escherichia coli is refolded by refolding buffer incubation overnight and gel filtration at 4°C, overview |
666821 |
| 3.1.1.3 | the intein tag is cleaved with dithiothreitol and the refolded lipase is obtained in active form (activity recovery of 80%) |
691095 |
| 3.1.1.3 | through heating at 80°C for 30 min in the presence of 6 M Gdm-HCl and 1 M NaCl |
694965 |
