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Literature summary for 3.1.1.3 extracted from

  • Quyen, D.T.; Le, T.T.G.; Nguyen, T.T.; Oh, T.K.; Lee, J.K.
    High-level heterologous expression and properties of a novel lipase from Ralstonia sp. M1 (2005), Protein Expr. Purif., 39, 97-106.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Dichloromethane 55% activation of recombinant refolded enzyme at 30% v/v, 67% at 10% v/v Ralstonia sp.
dimethylformamide 28% activation of recombinant refolded enzyme at 10% v/v, 57% inhibition of recombinant refolded enzyme at 30% v/v Ralstonia sp.
DMSO 64% activation of recombinant refolded enzyme at 10% v/v, no effect at 30% v/v Ralstonia sp.
ethanol 43% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v Ralstonia sp.
ethanolamine 234% activation of recombinant refolded enzyme at 30% v/v, 37% at 10% v/v Ralstonia sp.
ethyl acetate 98% activation of recombinant refolded enzyme at 30% v/v Ralstonia sp.
hexane 53% activation of recombinant refolded enzyme at 10-30% v/v Ralstonia sp.
Isopropanol 39% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v Ralstonia sp.
additional information organic solvents induce the enzyme Ralstonia sp.
SDS 83% inhibition of the recombinant refolded enzyme at 5% w/v, 52% activation of the recombinant refolded enzyme at 1% w/v Ralstonia sp.
tert-butanol 41% activation of recombinant refolded enzyme at 30% v/v Ralstonia sp.
Triton X-100 19-39% activation of the recombinant refolded enzyme at 0.2-1% w/v, 89% inhibition of the recombinant refolded enzyme at 5% w/v Ralstonia sp.
Triton X-45 42-45% activation of the recombinant refolded enzyme at 0.2-1% w/v, 31% inhibition of the recombinant refolded enzyme at 5% w/v Ralstonia sp.
Tween 40 recombinant refolded enzyme Ralstonia sp.
Tween 60 recombinant refolded enzyme Ralstonia sp.
Tween 80 recombinant refolded enzyme Ralstonia sp.

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of LipA and a 56-amino acid truncated chaperone DELTALipBHis6 in Escherichia coli strain Bl21, LIpA in expressed in inclusion bodies Ralstonia sp.

Inhibitors

Inhibitors Comment Organism Structure
1-butanol 47% inhibition of recombinant refolded enzyme at 10% v/v Ralstonia sp.
2-mercaptoethanol maximal inhibition of 32% at 1 mM of recombinant refolded enzyme Ralstonia sp.
acetone 45% inhibition of recombinant refolded enzyme at 30% v/v Ralstonia sp.
acetonitrile 60% inhibition of recombinant refolded enzyme at 30% v/v Ralstonia sp.
Ca2+ 14% activation of recombinant refolded enzyme at 0.1 mM, 17% inhibition at 10 mM Ralstonia sp.
diethyldicarbonate 15% inhibition of recombinant refolded enzyme at 10 mM Ralstonia sp.
dimethylformamide 57% inhibition of recombinant refolded enzyme at 30% v/v, 28% activation of recombinant refolded enzyme at 10% v/v Ralstonia sp.
EDTA 27-29% inhibition of recombinant refolded enzyme at 0.1-10 mM Ralstonia sp.
ethanol 43% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v Ralstonia sp.
Fe3+ 14% inhibition of recombinant refolded enzyme at 10 mM, 23% at 1 mM, 18% at 0.1 mM Ralstonia sp.
Isopropanol 39% activation of recombinant refolded enzyme at 10% v/v, and 44% inhibition at 30% v/v Ralstonia sp.
Mg2+ 10% activation of recombinant refolded enzyme at 1 mM, 15% inhibition at 10 mM Ralstonia sp.
PMSF 43% inhibition of recombinant refolded enzyme at 10 mM, 15% at 1 mM Ralstonia sp.
SDS 52% activation of the recombinant refolded enzyme at 1% w/v, 83% inhibition of the recombinant refolded enzyme at 5% w/v Ralstonia sp.
Triton X-100 19-39% activation of the recombinant refolded enzyme at 0.2-1% w/v, 89% inhibition of the recombinant refolded enzyme at 5% w/v Ralstonia sp.
Triton X-45 42-45% activation of the recombinant refolded enzyme at 0.2-1% w/v, 31% inhibition of the recombinant refolded enzyme at 5% w/v Ralstonia sp.
Zn2+ 70% inhibition of recombinant refolded enzyme at 10 mM, 66% at 1 mM, 35% at 0.1 mM Ralstonia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 14% activation of recombinant refolded enzyme at 0.1 mM, 17% inhibition at 10 mM Ralstonia sp.
Cu2+ no activation of recombinant refolded enzyme at 10 mM and 1 mM, 14% at 0.1 mM Ralstonia sp.
Mg2+ 10% activation of recombinant refolded enzyme at 1 mM, 15% inhibition at 10 mM Ralstonia sp.
Mn2+ 22% activation of recombinant refolded enzyme at 10 mM Ralstonia sp.
Sn2+ no activation of recombinant refolded enzyme at 10 mM and 1 mM, 12% at 0.1 mM Ralstonia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
x * 32500, recombinant enzyme Ralstonia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
triacylglycerol + H2O Ralstonia sp. of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview diacylglycerol + a carboxylate
-
?
triacylglycerol + H2O Ralstonia sp. M1 of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview diacylglycerol + a carboxylate
-
?
tributyrin + H2O Ralstonia sp.
-
dibutyrin + butyrate
-
?
tributyrin + H2O Ralstonia sp. M1
-
dibutyrin + butyrate
-
?
tricaprylin + H2O Ralstonia sp.
-
dicaprylin + caprylate
-
?
triolein + H2O Ralstonia sp. olive oil diolein + oleate
-
?
triolein + H2O Ralstonia sp. M1 olive oil diolein + oleate
-
?

Organism

Organism UniProt Comment Textmining
Ralstonia sp.
-
gene lipA
-
Ralstonia sp. M1
-
gene lipA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type LipA and a 56-amino acid truncated chaperone DELTALipBHis6 from Escherichia coli strain BL21 by nickel affinity chromatography Ralstonia sp.

Renatured (Commentary)

Renatured (Comment) Organism
refolding of wild-type LipA from inclusion bodies solublized by 8 M urea, refolded with a 56-amino acid truncated chaperone DELTALipBHis6 at 4°C for 24 h in water in a ratio of 1:1 Ralstonia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl caprylate + H2O preferred 4-nitrophenyl substrate of the recombinant enzyme Ralstonia sp. 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O preferred 4-nitrophenyl substrate of the recombinant enzyme Ralstonia sp. M1 4-nitrophenol + caprylate
-
?
4-nitrophenyl myristate + H2O preferred 4-nitrophenyl substrate of the wild-type enzyme Ralstonia sp. 4-nitrophenol + myristate
-
?
additional information substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids Ralstonia sp. ?
-
?
additional information substrate specificity of the recombinant and the wild-type enzyme, preference for triacylglycerides with short length fatty acids Ralstonia sp. M1 ?
-
?
triacylglycerol + H2O of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview Ralstonia sp. diacylglycerol + a carboxylate
-
?
triacylglycerol + H2O of several natural oils, e.g. cottonseed oil, corn oil, palm oil, wheatgerm oil, soybean oil, rapeseed oil, coconut oil, linseed oil, peanut oil, and olive oil in descending activity, overview Ralstonia sp. M1 diacylglycerol + a carboxylate
-
?
tributyrin + H2O
-
Ralstonia sp. dibutyrin + butyrate
-
?
tributyrin + H2O
-
Ralstonia sp. M1 dibutyrin + butyrate
-
?
tricaprylin + H2O
-
Ralstonia sp. dicaprylin + caprylate
-
?
triolein + H2O olive oil Ralstonia sp. diolein + oleate
-
?
triolein + H2O olive oil Ralstonia sp. M1 diolein + oleate
-
?

Subunits

Subunits Comment Organism
? x * 32500, recombinant enzyme Ralstonia sp.

Synonyms

Synonyms Comment Organism
LipA
-
Ralstonia sp.
lipase
-
Ralstonia sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55 recombinant refolded enzyme Ralstonia sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 75 about 20% of maximal activity at 25°C, 60% at 75°C, recombinant refolded enzyme Ralstonia sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
stable up to, recombinant refolded enzyme Ralstonia sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.8
-
recombinant refolded enzyme, narrow optimum Ralstonia sp.

pH Range

pH Minimum pH Maximum Comment Organism
8.5 11.5 about 20% of maximal activity at pH 10.0 and pH 11.0, recombinant refolded enzyme Ralstonia sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
8 10.5 52% remaining activity, recombinant refolded enzyme Ralstonia sp.