EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
6.3.5.4 | 0.45 |
- |
L-Gln |
mutant E348Q, glutaminase activity, pH 8.0, 37°C, ATP absent |
714228 |
6.3.5.4 | 0.63 |
- |
L-Asp |
wild-type, ammonia-dependent activity, pH 8.0, 37°C |
714228 |
6.3.5.4 | 0.63 |
- |
L-Asp |
wild-type, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C |
714228 |
6.3.5.4 | 1.1 |
- |
L-Gln |
mutant E348A, glutaminase activity, pH 8.0, 37°C, ATP absent |
714228 |
6.3.5.4 | 1.14 |
- |
L-Gln |
mutant E348Q, glutaminase activity, pH 8.0, 37°C, 5 mM ATP present |
714228 |
6.3.5.4 | 1.19 |
- |
L-Gln |
wild-type, glutaminase activity, pH 8.0, 37°C, ATP absent |
714228 |
6.3.5.4 | 1.2 |
- |
L-Asp |
wild-type, glutamine-dependent activity, pH 8.0, 37°C |
714228 |
6.3.5.4 | 1.2 |
- |
L-Asp |
wild-type, synthetase activity, glutamine-dependent activity, 20 mM L-Gln, pH 8.0, 37°C |
714228 |
6.3.5.4 | 1.3 |
- |
L-Asp |
mutant E348D, ammonia-dependent activity, pH 8.0, 37°C |
714228 |
6.3.5.4 | 1.3 |
- |
L-Asp |
mutant E348D, synthetase activity, ammonia-dependent activity, 100 mM NH4Cl, pH 8.0, 37°C |
714228 |