EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
2.7.1.21 | 0.0019 |
- |
deoxycytidine |
mutant enzyme L134A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.0024 |
- |
beta-L-thymidine |
at pH 7.8 and 25°C |
722671 |
2.7.1.21 | 0.0028 |
- |
thymidine |
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.0031 |
- |
3'-azido-3'-deoxythymidine |
mutant enzyme T163S, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.0056 |
- |
deoxyadenosine |
full-length enzyme, at pH 7.8 and 37°C |
722758 |
2.7.1.21 | 0.0066 |
- |
deoxyguanosine |
mutant enzyme S182A, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.0067 |
- |
3'-azido-3'-deoxythymidine |
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.0068 |
- |
thymidine |
wild type enzyme, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |
2.7.1.21 | 0.03 |
- |
uridine |
pH 7.4, 25°C |
739404 |
2.7.1.21 | 0.048 |
- |
deoxycytidine |
mutant enzyme M42I, in 50 mM Tris-HCl, pH 7.5, 10 mM dithiothreitol, 2.5 mM MgCl2, 0.5 mM CHAPS, 3 mM NaF, 0.5 mg/ml bovine serum albumin and 2.5 mM ATP, temperature not specified in the publication |
722240 |