EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    2.5.1.47 | -999 |
- |
more |
wild type and mutant exhibit almost identical values for sulfide as substrate |
684707 |
| 2.5.1.47 | 0.01 |
- |
O3-acetyl-L-serine |
K120Q mutant with 3.7fold decrease compared to wild type value, 100 mM MES, pH 6.5, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C |
685397 |
| 2.5.1.47 | 0.0219 |
- |
5-thio-2-nitrobenzoate |
H152A mutant with 43fold decrease compared to wild type, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C |
684707 |
| 2.5.1.47 | 0.033 |
- |
thiosulfate |
30°C, pH 8.0 |
721306 |
| 2.5.1.47 | 0.037 |
- |
O3-acetyl-L-serine |
wild type, 100 mM HEPES, pH 7.0, 50 microM 5-thio-2-nitrobenzoate (TNB), 25°C |
684707, 685397 |
| 2.5.1.47 | 0.1 |
- |
O-acetyl-L-serine |
production of H2S, mutant A72S, pH 8.2, 37°C |
747747 |
| 2.5.1.47 | 0.11 |
- |
O-acetyl-L-serine |
production of H2S, wild-type, pH 8.2, 37°C |
747747 |
| 2.5.1.47 | 0.14 |
- |
O-acetyl-L-serine |
30°C, pH 8.0, cosubstrate: hydrogen sulfide |
721306 |
| 2.5.1.47 | 0.141 |
- |
5-thio-2-nitrobenzoate |
K120Q mutant with 6.7fold decrease compared to wild type value, 100 mM MES, pH 6.5, 0.5 mM O3-acetyl-L-serine, 25°C |
685397 |
| 2.5.1.47 | 0.15 |
- |
O-acetyl-L-serine |
mutant F143D |
704338 |
