EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    1.4.1.9 | 0.0025 |
- |
2-oxobutanoate |
wild-type, pH 7.5, 37°C |
741659 |
| 1.4.1.9 | 0.004 |
- |
trimethylpyruvate |
wild-type, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.005 |
- |
trimethylpyruvate |
mutant T45M, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.014 |
- |
trimethylpyruvate |
mutant T45M/E116V, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.019 |
- |
oxo(phenyl)acetate |
wild-type, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.019 |
- |
trimethylpyruvate |
mutant E116V, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.0217 |
- |
2-oxobutanoate |
mutant M374G, pH 7.5, 37°C |
741659 |
| 1.4.1.9 | 0.022 |
- |
2-oxobutanoate |
mutant M347G, pH 7.5, 37°C |
741659 |
| 1.4.1.9 | 0.03 |
- |
oxo(phenyl)acetate |
mutant T45M, pH 9.5, 35°C |
762851 |
| 1.4.1.9 | 0.0492 |
- |
2-oxobutanoate |
mutant M347G/Q358N, pH 7.5, 37°C |
741659 |
