EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.286 | 157.1 |
- |
homoisocitrate |
pH 8.0, 60°C, recombinant enzyme |
740000 |
1.1.1.286 | 262.1 |
- |
isocitrate |
pH 8.0, 60°C, recombinant enzyme |
740000 |
1.1.1.286 | 2259 |
- |
isocitrate |
pH 8.0, 60°C, recombinant enzyme |
738195 |
1.1.1.286 | 3364 |
- |
homoisocitrate |
pH 8.0, 60°C, recombinant enzyme |
738195 |
1.1.1.286 | 0.45 |
- |
isocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N |
740021 |
1.1.1.286 | 98 |
- |
homoisocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N |
740021 |
1.1.1.286 | 70 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with homoisocitrate |
740021 |
1.1.1.286 | 0.56 |
- |
NAD+ |
pH 8.0, 60°C, recombinant His6-tagged mutant I82N, with isocitrate |
740021 |
1.1.1.286 | 0.92 |
- |
isocitrate |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P |
740021 |
1.1.1.286 | 2.2 |
- |
3-isopropylmalate |
pH 8.0, 60°C, recombinant His6-tagged mutant L81P |
740021 |