EC Number   |
General Information |
Reference  |
|---|
   3.5.1.105 | evolution |
the enzyme belongs to the carbohydrate esterase family 4. Amino acid residues that officiate as the metal ion-binding triad and general acidbase catalysts are generally conserved in other Zn-dependent deacetylases |
-, 733875 |
| 3.5.1.105 | more |
His291 and Asp35, which are in the vicinity of the zinc ion-binding triad, act as the catalytic base and acid, respectively. The enzyme comprises one polysaccharide deacetylase domain and two carbohydrate-binding domains. The carbohydrate-binding domains are unlikely to affect the configuration of the active center residues in active site of polysaccharide deacetylase domain, overview |
-, 733875 |
| 3.5.1.105 | physiological function |
the enzyme generates beta-N-acetyl-D-glucosaminyl-(1,4)-D-glucosamine from (GlcNAc)2, in strain KN1699, GlcNAc-GlcN is an end product of chitin degradation outside the cell |
-, 733875 |
| 3.5.1.105 | evolution |
the enzyme is an evolutionarily conserved protein that belongs to the the YdjC family of proteins |
-, 734074 |
| 3.5.1.105 | metabolism |
deacetylated chitobiose and chitotriose provide the induction signal necessary for the activation of the ChbR regulator |
-, 734074 |
| 3.5.1.105 | more |
three conserved residues Asp11, His61, and His125 form the catalytic triad |
-, 734074 |
| 3.5.1.105 | physiological function |
the monodeacetylase that is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Activation of the chb promoter by the regulatory protein ChbR is dependent on ChbG, deacetylation of chitobiose-6-phosphate and chitotriose-6-phosphate is necessary for their recognition by ChbR as inducers. ChbR-independent expression of the permease and phospho-beta-glucosidase from a heterologous promoter did not support growth on both chitobiose and chitotriose in the absence of chbG, suggesting an additional role of chbG in the hydrolysis of chitooligosaccharides |
-, 734074 |
| 3.5.1.105 | evolution |
the enzyme belongs to carbohydrate esterase (CE) family 14 |
-, 745652 |
| 3.5.1.105 | metabolism |
N,N'-diacetylchitobiose deacetylase (Dac) belongs to the CE-14 family and plays a role in the chitinolytic pathway in archaea by deacetylating N,N'-diacetylchitobiose (GlcNAc2), which is the end product of chitinase |
-, 745652 |
| 3.5.1.105 | more |
enzyme structure analysis and modelling, molecular dynamics simulation |
-, 745652 |
