EC Number   |
General Information |
Reference |
|---|
  1.14.14.180 | evolution |
in a phylogenetic tree, tomato CYP85A3 is placed on the same branch as the CYP85A family of tomato, Arabidopsis, and rice, and is closest to tomato CYP85A1 |
765036 |
| 1.14.14.180 | physiological function |
brassinolide is not identified in Brachypodium distachyon. Crude enzyme solutions cannot catalyze the conversion of castasterone to brassinolide, and the gene encoding an ortholog of CYP85A2 is not found |
764957 |
| 1.14.14.180 | physiological function |
CYP85A2 catalyzes the lactonization reactions of castasterone and also teasterone and typhasterol. A Pichia pastoris transformant that synchronously expresses Arabidopsis thaliana P450 reductase gene ATR1 and P450 gene CYP85A2 converts teasterone and typhasterol to 7-oxateasterone and 7-oxatyphasterol, respectively |
764322 |
| 1.14.14.180 | physiological function |
CYP85A2 shows castasterone synthase and also brassinolide synthase activity. Castasterone is the major active brassinosteroid during vegetative growth in tomato, whereas brassinolide may play an organ-specific role in fruit development. CYP85A2 T-DNA insertion mutants consistently exhibit a weak dwarf phenotype during early vegetative development and a reduced fertility after bolting. CYP85A1/CYP85A2 double mutants grow as severe dwarfs |
765036 |
| 1.14.14.180 | physiological function |
enzyme is a cytochrome P450 that mediates Baeyer-Villiger oxidation in plants. Castasterone isa bioactive brassinosteroid that controls the overall growth and development of Arabidopsis plants. Mutant studies also revealed that brassinolide may not always be necessary for normal growth and development but Arabidopsis plants acquire great benefit in terms of growth and development in the presence of brassinolide |
765553 |
| 1.14.14.180 | physiological function |
overexpression in seeds increases the levels of castasterone and brassinolide in seeds. Compared to the wild type, the overexpressing strains produces substantially larger seeds with a high concentration of nutrients due to an enhancement in brassinosteroids signaling. Additionally, it exhibits superior seed germination, seedling and rosette plant growth, and flower and silique formation |
765274 |
| 1.14.14.180 | physiological function |
seeds of transgenic Brachypodium distachyon expressing CYP85A2 synthesize castasterone. In comparison with wild-type B. distachyon, the transgenic plants show better growth and development during the vegetative growing stage. The flowers of the transgenic plants are remarkably larger, resulting in increments in the number, size, and height of seeds. The total starch, protein, and lipid contents in transgenic plants are higher than those in wild-type plants |
764786 |
