EC Number   |
General Information |
Reference |
|---|
    2.8.1.11 | physiological function |
the E1-catalyzed activation of the ubiquitin-like protein resembles the second step of the molybdenum cofactor (Moco) biosynthesis in humans and bacteria. For Moco biosynthesis in humans, the E1-like protein MOCS3 forms a thiocarboxylate group at the C-terminal glycine of the beta-grasp fold protein MOCS2A. molybdenum cofactor biosynthesis and tRNA thiolation steps are linked by the MOCS3 protein in humans, mechanism of protein conjugation and thiocarboxylate formation in sulfur transfer pathways, overview |
722756 |
| 2.8.1.11 | physiological function |
the human MOCS3 gene encodes a protein involved in activation and sulfuration of the C-terminus of MOCS2A, the smaller subunit of the molybdopterin (MPT) synthase. MPT synthase catalyzes the formation of the dithiolene group of MPT that is required for the coordination of the molybdenum atom in the last step of molybdenum cofactor (Moco) biosynthesis. The L-cysteine desulfurase Nfs1 might be involved in the sulfuration ofMOCS3 in vivo. Sulfur transfer mechanism, overview |
722660 |
| 2.8.1.11 | physiological function |
the human MOCS3 protein contains a C-terminal segment displaying similarities to the sulfurtransferase rhodanese. MOCS3 catalyzes both the adenylation and the subsequent generation of a thiocarboxylate group at the C-terminus of the smaller subunit of molybdopterin, MPT, synthase during molybdenum cofactor biosynthesis in humans. The N-terminus of MOCS3 is expected to activate the C-terminal glycine of, MOCS2A to form an acyl adenylate. Subsequently, the C-terminal rhodanese-like domain (RLD) of MOCS3 acts as a direct sulfur donor for the formation of a thiocarboxylate group on MOCS2A, The MOCS2A thiocarboxylate sulfur is used for the generation of the dithiolene moiety of molybdopterin which coordinates the molybdenum atom in molybdenum cofactor. The enzyme is able to provide the sulfur for the thiocarboxylation of MOCS2A in a defined in vitro system for the generation of MPT from precursor Z |
721600 |
| 2.8.1.11 | physiological function |
the MOCS3 protein is believed to catalyze both the adenylation and the subsequent generation of a thiocarboxylate group at the C terminus of the smaller subunit of molybdopterin (MPT) synthase, the C-terminal segment of MOCS3 displays similarities to the sulfurtransferase rhodanese. The MOCS3 rhodanese-like domain provides the sulfur for the thiocarboxylation of MOCS2A, the small MPT synthase subunit in humans. C412 is important for catalysis. The MoeB domain of MOCS3 is not involved in sulfur transfer in vitro |
723621 |
