EC Number |
Reaction |
Reference |
---|
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
bi bi uni uni ping pong mechanism, with glutamyl-enzyme as intermediate before the addition of cys and the release of gamma-glutamylcysteine |
1135 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
Cys553 is not the active site residue |
649655 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
enzyme-bound reaction intermediate is a gamma-glutamyl-phosphate, active site cysteine, catalytic mechanism and substrate binding |
649263 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
enzyme-bound reaction intermediate is a gamma-glutamyl-phosphate, active site cysteine, catalytic mechanism, active site and substrate binding, Lys38 is an active site residue in the glutamyl binding site, His150 is essential for activity |
649263 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
enzyme-bound reaction intermediate is a gamma-glutamyl-phosphate, active site cysteine, mechanism |
649263 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
no conserved cysteine residue in the active site |
649263 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
partially random mechanism in which ATP is the obligatory first substrate and both amino acids bind in a random order to the enzyme*ATP complex. Formation of the enzyme*substrate quarternary complex is necessary prior to release of products |
1136 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
rapid equilibrium random ter-reactant mechanism, substrate binding modeling, active site model |
650135 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
rapid equilibrium random ter-reactant mechanism, substrate binding modeling, Cys319 is an active site cysteine not essential for activity |
651896 |
6.3.2.2 | ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine |
reaction and kinetic mechanism, modeling |
651896 |