Information on EC 6.3.2.2 - glutamate-cysteine ligase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
6.3.2.2
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RECOMMENDED NAME
GeneOntology No.
glutamate-cysteine ligase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxamide formation
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carboxylic acid amide formation
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Ligation
peptide bond ligation
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ergothioneine biosynthesis I (bacteria)
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glutathione biosynthesis
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homoglutathione biosynthesis
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glutathione metabolism
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Cysteine and methionine metabolism
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Glutathione metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
L-glutamate:L-cysteine gamma-ligase (ADP-forming)
Can use L-aminohexanoate in place of glutamate.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-64-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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D4N892
UniProt
Manually annotated by BRENDA team
mallard duck
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Manually annotated by BRENDA team
genes GCLC and GCLM encoding catalytic heavy chain subunit and modifier light chain subunits
C1KBE8, C1KBE7
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Bacterium cadaveris
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
genes I79_010621 and I79_022778 encoding catalytic and regulatory subunits
G3HIY9, G3IG96
UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
symbiotic sea anemone, genes gclC and gclM encoding the catalytic and the modifier subunits of the enzyme
UniProt
Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
gene gshA
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Manually annotated by BRENDA team
gene gshA
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Manually annotated by BRENDA team
strain ATCC8293, gene gshA
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Manually annotated by BRENDA team
strain ATCC8293, gene gshA
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Manually annotated by BRENDA team
no activity in Entamoeba histolytica
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Manually annotated by BRENDA team
no activity in Giardia sp.
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Manually annotated by BRENDA team
PCC 7120
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Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Pseudomonas schuylkilliensis
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Streptococcus agalactiae serotype V
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UniProt
Manually annotated by BRENDA team
Streptococcus agalactiae serotype V ATCC BAA-611 / 2603 V/R
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
gene gshA or slr0990
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Uniprot
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
malfunction
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + alpha-ethyl-L-glutamate + L-alpha-aminobutyrate
ADP + phosphate + alpha-ethyl-L-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + alpha-methyl-DL-glutamate + L-alpha-aminobutyrate
ADP + phosphate + alpha-methyl-DL-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + alpha-methyl-L-glutamate + L-alpha-aminobutyrate
ADP + phosphate + alpha-methyl-L-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + alpha-methylglutamate + L-Cys
ADP + phosphate + alpha-methylglutamyl-L-Cys
show the reaction diagram
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i.e. 2-amino-2-methylpentanedioate
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ATP + beta-aminoglutarate + L-Cys
ADP + phosphate + beta-aminoglutaryl-L-Cys
show the reaction diagram
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i.e. 3-aminopentanedioate
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ATP + beta-Glu + L-Cys
ADP + phosphate + beta-Glu-L-Cys
show the reaction diagram
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17.6% of the activity relative to L-Glu
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ATP + beta-glutamate + L-alpha-aminobutyrate
ADP + phosphate + beta-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + beta-methyl-DL-glutamate + L-alpha-aminobutyrate
ADP + phosphate + beta-methyl-DL-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + beta-methylglutamate + L-Cys
ADP + phosphate + beta-methylglutamyl-L-Cys
show the reaction diagram
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i.e. 2-amino-3-methylpentanedioate
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ATP + D-Glu + L-2-aminobutyrate
ADP + phosphate + gamma-D-Glu-L-alpha-aminobutyrate
show the reaction diagram
ATP + D-Glu + L-alpha-aminobutyrate
ADP + phosphate + gamma-D-Glu-L-alpha-aminobutyrate
show the reaction diagram
ATP + D-Glu + L-Cys
ADP + phosphate + D-Glu-L-Cys
show the reaction diagram
ATP + DL-alpha-aminoadipate + L-cysteine
ADP + phosphate + DL-aminoadipyl-L-cysteine
show the reaction diagram
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about 10% of the activity with L-glutamate
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?
ATP + DL-alpha-aminomethylglutarate + L-alpha-aminobutyrate
ADP + phosphate + DL-alpha-aminomethylglutaryl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + DL-alpha-aminomethylsuccinate + L-alpha-aminobutyrate
ADP + phosphate + DL-alpha-aminomethylsuccinyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + DL-beta-aminoadipate + L-alpha-aminobutyrate
ADP + phosphate + DL-beta-aminoadipyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + glutamate + L-cysteine
ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
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random ter-reactant mechanism with a preferred binding order
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?
ATP + L-Glu
ADP + phosphate + 5-oxoproline
show the reaction diagram
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ir
ATP + L-Glu + (R)-beta-amino-iso-butyrate
ADP + phosphate + gamma-L-Glu-(R)-beta-amino-iso-butyrate
show the reaction diagram
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2fold less reactive as the S-isomer
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ir
ATP + L-Glu + (S)-beta-amino-iso-butyrate
ADP + phosphate + gamma-L-Glu-(S)-beta-amino-iso-butyrate
show the reaction diagram
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2fold as reactive as the R-isomer
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ir
ATP + L-Glu + allo-L-threonine
ADP + phosphate + gamma-L-Glu-allo-L-threonine
show the reaction diagram
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ir
ATP + L-Glu + beta-amino-iso-butyrate
ADP + phosphate + gamma-L-Glu-beta-amino-iso-butyrate
show the reaction diagram
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ir
ATP + L-Glu + beta-chloro-L-Ala
ADP + phosphate + gamma-L-Glu-L-beta-chloro-L-Ala
show the reaction diagram
ATP + L-Glu + beta-chloro-L-alanine
ADP + phosphate + gamma-L-Glu-beta-chloro-L-alanine
show the reaction diagram
ATP + L-Glu + beta-cyano-L-alanine
ADP + phosphate + gamma-L-Glu-beta-cyano-L-alanine
show the reaction diagram
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ir
ATP + L-Glu + butylamine
ADP + phosphate + N-butyl-L-glutamine
show the reaction diagram
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?
ATP + L-Glu + D-Cys
ADP + phosphate + gamma-L-Glu-D-Cys
show the reaction diagram
ATP + L-Glu + DL-allylglycine
ADP + phosphate + gamma-L-Glu-DL-allylglycine
show the reaction diagram
ATP + L-Glu + DL-beta-amino-iso-butyrate
ADP + phosphate + gamma-L-Glu-DL-beta-amino-iso-butyrate
show the reaction diagram
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ir
ATP + L-Glu + ethylamine
ADP + phosphate + N-ethyl-L-glutamine
show the reaction diagram
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?
ATP + L-Glu + gamma-aminobutyrate
ADP + phosphate + gamma-L-Glu-gamma-aminobutyrate
show the reaction diagram
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mutant R366A
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r
ATP + L-Glu + gamma-aminobutyrate
ADP + phosphate + L-Glu-gamma-aminobutyrate
show the reaction diagram
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ir
ATP + L-Glu + Gly
ADP + phosphate + gamma-L-Glu-Gly
show the reaction diagram
ATP + L-Glu + hydroxylamine
ADP + phosphate + gamma-L-Glu-hydroxylamine
show the reaction diagram
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slow reaction rate
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ATP + L-Glu + L-2-aminobutanoate
ADP + phosphate + gamma-L-Glu-2-aminobutanoate
show the reaction diagram
ATP + L-Glu + L-2-aminobutyrate
ADP + phosphate + gamma-L-Glu-2-aminobutyrate
show the reaction diagram
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?
ATP + L-Glu + L-2-aminobutyrate
ADP + phosphate + L-Glu-2-aminobutyrate
show the reaction diagram
ATP + L-Glu + L-Ala
ADP + phosphate + gamma-L-Glu-L-Ala
show the reaction diagram
ATP + L-Glu + L-alanine
ADP + phosphate + gamma-L-Glu-L-alanine
show the reaction diagram
ATP + L-Glu + L-alpha-aminobutyrate
ADP + phosphate + gamma-L-Glu-L-alpha-aminobutyrate
show the reaction diagram
ATP + L-Glu + L-alpha-aminoheptanoate
ADP + phosphate + gamma-L-Glu-L-alpha-aminoheptanoate
show the reaction diagram
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ir
ATP + L-Glu + L-C-allylglycine
ADP + phosphate + gamma-L-Glu-L-C-allylglycine
show the reaction diagram
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ir
ATP + L-Glu + L-Cys
?
show the reaction diagram
ATP + L-Glu + L-Cys
ADP + phosphate + gamma-L-Glu-L-Cys
show the reaction diagram
ATP + L-Glu + L-homocysteine
ADP + phosphate + gamma-L-Glu-L-homocysteine
show the reaction diagram
ATP + L-Glu + L-homoserine
ADP + phosphate + gamma-L-Glu-L-homoserine
show the reaction diagram
ATP + L-Glu + L-isoleucine
ADP + phosphate + gamma-L-Glu-L-isoleucine
show the reaction diagram
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ir
ATP + L-Glu + L-leucine
ADP + phosphate + gamma-L-Glu-L-leucine
show the reaction diagram
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ir
ATP + L-Glu + L-norleucine
ADP + phosphate + gamma-L-Glu-L-norleucine
show the reaction diagram
ATP + L-Glu + L-norvaline
ADP + phosphate + gamma-L-Glu-L-norvaline
show the reaction diagram
ATP + L-Glu + L-Ser
ADP + phosphate + gamma-L-Glu-L-Ser
show the reaction diagram
ATP + L-Glu + L-serine
ADP + phosphate + gamma-L-Glu-L-serine
show the reaction diagram
ATP + L-Glu + L-Thr
ADP + phosphate + gamma-L-Glu-L-Thr
show the reaction diagram
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ATP + L-Glu + L-threonine
ADP + phosphate + gamma-L-Glu-L-threonine
show the reaction diagram
ATP + L-Glu + L-valine
ADP + phosphate + gamma-L-Glu-L-valine
show the reaction diagram
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ir
ATP + L-Glu + methylamine
ADP + phosphate + N-methyl-L-glutamine
show the reaction diagram
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?
ATP + L-Glu + n-propylamine
ADP + phosphate + N-propyl-L-glutamine
show the reaction diagram
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?
ATP + L-Glu + O-methyl-DL-serine
ADP + phosphate + gamma-L-Glu-O-methyl-DL-serine
show the reaction diagram
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ir
ATP + L-Glu + S-methyl-L-Cys
ADP + phosphate + gamma-L-Glu-L-S-methyl-Cys
show the reaction diagram
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ir
ATP + L-Glu + S-methyl-L-Cys
ADP + phosphate + gamma-L-Glu-S-methyl-L-Cys
show the reaction diagram
ATP + L-Glu + S-methyl-L-cysteine
ADP + phosphate + gamma-L-Glu-S-methyl-L-cysteine
show the reaction diagram
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ir
ATP + L-glutamate + 2-aminobutyrate
ADP + phosphate + gamma-L-glutamyl-(2-aminobutyrate)
show the reaction diagram
ATP + L-glutamate + L-cysteine
ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
ATP + L-glutamate + L-cysteine
ADP + phosphate + L-glutamyl-L-cysteine
show the reaction diagram
ATP + N-methyl-L-glutamate + L-alpha-aminobutyrate
ADP + phosphate + N-methyl-L-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
ATP + N-methyl-L-glutarate + L-Cys
ADP + phosphate + N-methyl-L-glutaryl-L-Cys
show the reaction diagram
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ATP + threo-beta-hydroxy-DL-glutamate + L-alpha-aminobutyrate
ADP + phosphate + threo-beta-hydroxy-DL-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
ATP + threo-beta-hydroxy-L-Glu + L-Cys
ADP + phosphate + threo-beta-hydroxy-L-Glu-L-Cys
show the reaction diagram
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ATP + threo-gamma-hydroxy-L-glutamate + L-alpha-aminobutyrate
ADP + phosphate + threo-gamma-hydroxy-L-glutamyl-L-alpha-aminobutyrate
show the reaction diagram
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ir
glutamate + ATP + L-cysteine
ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
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assay at pH 8.2
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?
GTP + L-Glu + L-Cys
GDP + phosphate + gamma-L-Glu-L-Cys
show the reaction diagram
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87% of the activity relative to ATP
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L-glutamate + L-cysteine + ATP
ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
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?
UTP + L-Glu + L-Cys
UDP + phosphate + gamma-L-Glu-L-Cys
show the reaction diagram
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12% of the activity relative to ATP
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
ATP + L-Glu + L-Cys
?
show the reaction diagram
ATP + L-Glu + L-Cys
ADP + phosphate + gamma-L-Glu-L-Cys
show the reaction diagram
ATP + L-glutamate + L-cysteine
ADP + phosphate + gamma-L-glutamyl-L-cysteine
show the reaction diagram
ATP + L-glutamate + L-cysteine
ADP + phosphate + L-glutamyl-L-cysteine
show the reaction diagram
additional information
?
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