EC Number   |
Reaction |
Reference |
|---|
   4.6.1.24 | [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + H2O = [RNA fragment]-3'-guanosine-3'-phosphate |
(1b) |
- |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
hydrolysis of the phosphodiester bonds of single-stranded RNA at the 3'-side of guanosine nucleotides occurs with high specificity |
654052 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
mechanism |
24191, 24200 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
overall reaction |
- |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
small single-domain protein of 104 amino acid residues that cleaves single-stranded RNA specifically at the 3'-side of guanine residues in a two-step mechanism |
654917 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
the conjugate base of the gamma-carboxyl group of Clu46 is required for tight binding of guanine and hypoxanthine moieties at the primary recognition site of the enzyme |
654350 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
the enzyme is composed of 104 amino acid residues and it cleaves single-stranded RNA specifically after guanosine residues to produce mono or oligo-ribonucleotides with terminal 3'-phosphate |
654991 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
the reaction is a transphosphorylation yielding a 2',3'-cyclophosphate. This cyclic intermediate is hydrolyzed by a water molecule to a 3'-phosphate in a separate step which is the reversal of the transphosphorylation. The transphosphorylation consists of a nucleophilic in-line inversion displacement at the phosphorous atom of the 5'-leaving group by the entering 2'-oxygen. Tyr38, His40, Glu58, His92 and Phe100 are involved in the catalysis of RNA degradation. During cyclization, Glu58 and His92 serve as the catalytic base and acid, respectively |
655200 |
| 4.6.1.24 | [RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
the side chain carboxyl of Glu54 accepts a proton from the ribose 2'OH group and the protonated imidazole ring of His85 donates a proton to the leaving 5'O group. The positively charged side chain of Arg65 is to promote the formation of a negatively charged, pentacovalent intermediate state of the phosphate group |
657297 |
| 4.6.1.24 | [RNA] containing guanosine = [RNA fragment]-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] |
(1a) |
- |
