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Literature summary for 4.6.1.24 extracted from

  • Jo Chitester, B.; Walz, F.G., Jr.
    Kinetic studies of guanine recognition and a phosphate group subsite on ribonuclease T1 using substitution mutants at Glu46 and Lys41 (2002), Arch. Biochem. Biophys., 406, 73-77.
    View publication on PubMed
Search for more literature for 4.6.1.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Epicurian coli Aspergillus oryzae

Protein Variants

Protein Variants Comment Organism
K41M mutant with identical catalytic properties as the wild type enzyme with guanosine-2',3'-cyclic monophosphate as substrate, but less active with polyinosinic acid as substrate Aspergillus oryzae
K41T mutant with identical catalytic properties as the wild type enzyme with guanosine-2',3'-cyclic monophosphate as substrate, but less active with polyinosinic acid as substrate Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1530
-
 polyinosinic acid pH 6.0, 25ºC, wild type enzyme Aspergillus oryzae
1670
-
 guanosine-2',3'-cyclic monophosphate pH 6.0, 25ºC, wild type enzyme Aspergillus oryzae
1730
-
 guanosine-2',3'-cyclic monophosphate pH 6.0, 25ºC, K41M mutant Aspergillus oryzae
2010
-
 guanosine-2',3'-cyclic monophosphate pH 6.0, 25ºC, K41T mutant Aspergillus oryzae
3040
-
 polyinosinic acid pH 6.0, 25ºC, K41M mutant Aspergillus oryzae
3060
-
 polyinosinic acid pH 6.0, 25ºC, K41T mutant Aspergillus oryzae

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography and gel filtration Aspergillus oryzae

Reaction

Reaction Comment Organism Reaction ID
[RNA] containing guanosine + H2O = an [RNA fragment]-3'-guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA fragment] the conjugate base of the gamma-carboxyl group of Clu46 is required for tight binding of guanine and hypoxanthine moieties at the primary recognition site of the enzyme Aspergillus oryzae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanosine-2',3'-cyclic phosphate + H2O hydrolysis Aspergillus oryzae guanosine 3'-phosphate
-
 ?
guanylyl-3',5'-cytidine Transphosphorylation reaction Aspergillus oryzae guanosine-2',3'-cyclic phosphate + cytidine
-
 ?
polyinosinic acid + H2O polyI Aspergillus oryzae ?
-
 ?