    2.4.1.83 | GDP-alpha-D-mannose + dolichyl phosphate = GDP + dolichyl beta-D-mannosyl phosphate |
catalytic mechanism of PfDPMS, overview. Catalytic mechanism of the transfer reaction, nucleophilic attack by the Dol-P phosphate oxygen on the mannosyl C1 carbon yields GDP and Dol-P-Man. Asp91 and Gln93 coordinate the GDP-Man diphosphate groups via the metal ion, while the key side chains for positioning the Dol-P phosphate group for mannosyl transfer are Ser135 and Arg117. The Dol-P phosphate group is pre-activated for nucleophilic attack, and not dependent on a catalytic base for activation |
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