EC Number   |
Reaction |
Reference  |
|---|
   4.6.1.18 | an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
(1) overall reaction |
- |
| 4.6.1.18 | an [RNA] containing cytidine = an [RNA]-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
(1a) |
- |
| 4.6.1.18 | an [RNA] containing uridine + H2O = an [RNA]-3'-uridine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
(2) |
- |
| 4.6.1.18 | an [RNA] containing uridine = an [RNA]-3'-uridine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
(2a) |
- |
| 4.6.1.18 | an [RNA]-3'-cytidine-2',3'-cyclophosphate + H2O = an [RNA]-3'-cytidine-3'-phosphate |
(1b) |
- |
| 4.6.1.18 | an [RNA]-3'-uridine-2',3'-cyclophosphate + H2O = an [RNA]-3'-uridine-3'-phosphate |
(2b) |
- |
| 4.6.1.18 | an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
the catalytic site of the enzyme is mainly composed of His12, His119, Gln11, Lys41 and Asp121. The conformation of Lys41 and Gln11 are pH dependent and their occurrence is correlated to the release of a sulfate ion from the active site at neutral pH. The sulfate anion is progressively released with pH and water molecules occupy the active site at pH 7.1. The enzyme adopts different conformational states in different pH conditions. In the protonated state, Lys41 points towards the active site both in the presence and in the absence of sulfate and acts as a general base during catalysis |
654126 |
| 4.6.1.18 | an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
Gly38 and Glu111 are crucial for the catalytic activity |
654423 |
| 4.6.1.18 | an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
specifically located basic residues, together with the lack of a negative charge and/or the presence of a glycine at position 38, may contribute to make the enzymatic degradation of the polyanionic double-helical substrate more efficient |
654672 |
| 4.6.1.18 | an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] |
two isoforms of dimeric enzyme, D-I and D-II |
655538 |
