EC Number   |
|---|
   7.6.2.2 | - |
| 7.6.2.2 | anti-Flag M2 affinity agarose gel chromatography and Superdex 200 gel filtration |
| 7.6.2.2 | approach involving its specific recognition onto a functionalized lipid layer containing a Ni-NTA lipidand allowing the purification of His-ABCG2 in presence of all solubilized membrane components that might be involved in the stabilisation of native oligomers and without requiring any additional washing or concentration passages |
| 7.6.2.2 | co-purification of YdaG and YdbA |
| 7.6.2.2 | cobalt affinity resin column chromatography, GST column chromatography, and Superdex 200 gel filtration |
| 7.6.2.2 | cysteine-free mouse MDR3 P-glycoprotein (Pgp) is constructed by mutagenesis of the nine natural Cys to Ala, expressed in Pichia pastoris and purified |
| 7.6.2.2 | native enzyme from liver partially by mitochondria, plasma, endoplasmic reticulum and nuclear membrane fragment preparation |
| 7.6.2.2 | native P-gp by CHAPS-mediated extraction from plasma membrane, followed by concanavalin A affinity chromatography. Reconstitution in lipid proteoliposomes, that are further purified by gel filtration |
| 7.6.2.2 | Ni-NTA agarose column chromatography |
| 7.6.2.2 | Ni-NTA column chromatography |
